Trace analysis of polychlorinated dibenzo-p-dioxins, dibenzofurans and WHO polychlorinated biphenyls in food using comprehensive two-dimensional gas chromatography with electron-capture detection
2005 (English)In: Journal of Chromatography A, ISSN 0021-9673, Vol. 1086, no 1-2, 61-70 p.Article in journal (Refereed) Published
Trace analysis of 2,3,7,8-polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs) and the 12 WHO-PCBs (four non-ortho and eight mono-ortho congeners that have been assigned toxic equivalence factors, TEFs, by the World Health Organisation) was conducted by comprehensive two-dimensional gas chromatography with a micro electron-capture detector (GC x GC-mu ECD). Four food matrices (fish oil from herring, spiked cows' milk, vegetable oil and an eel extract) were analysed by two GC x GC laboratories, and four GC-HRMS laboratories generated reference values. The two GC x GC laboratories used different column combinations for separating the target analytes. For the first dimension, non-polar DB-XLB and VF-1 columns were used, and for the second dimension, an LC-50 liquid crystalline column with unique selectivity for planar compounds. The congener-specific and total toxic equivalence (TEQ) data obtained using DB-XLB x LC-50 were in good agreement with results obtained by the GC-HRMS laboratories. The WHO-PCB data obtained with the VF-1 x LC-50 combination was also good, but the PCDD/F concentrations were sometimes overestimated due to matrix interferences. GC x GC-mu ECD using DB-XLB x LC-50 seems to fulfil the European Community requirements of a screening method for PCDD/F and WHO-PCB TEQ in food.
Place, publisher, year, edition, pages
2005. Vol. 1086, no 1-2, 61-70 p.
comprehensive two-dimensional gas chromatography, GC x GC, mu ECD; PCB, PCDD/F, TEF, TEQ, quantification, food, Dioxins, GC-HRMS
IdentifiersURN: urn:nbn:se:umu:diva-13351DOI: doi:10.1016/j.chroma.2004.11.071OAI: oai:DiVA.org:umu-13351DiVA: diva2:153022