Isolation of Outer Membrane of Synechocystis sp. PCC 6803 and Its Proteomic Characterization
2004 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, Vol. 3, no 6, 586-95 p.Article in journal (Refereed) Published
In this report, we describe a newly developed method for isolating outer membranes from Synechocystis sp. PCC 6803 cells. The purity of the outer membrane fraction was verified by immunoblot analysis using antibodies against membrane-specific marker proteins. We investigated the protein composition of the outer membrane using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry followed by database identification. Forty-nine proteins were identified corresponding to 29 different gene products. All of the identified proteins have a putative N-terminal signal peptide. About 40% of the proteins identified represent hypothetical proteins with unknown function. Among the proteins identified are a Toc75 homologue, a protein that was initially found in the outer envelope of chloroplasts in pea, as well as TolC, putative porins, and a pilus protein. Other proteins identified include ABC transporters and GumB, which has a suggested function in carbohydrate export. A number of proteases such as HtrA were also found in the outer membrane of Synechocystis sp. PCC 6803.
Place, publisher, year, edition, pages
2004. Vol. 3, no 6, 586-95 p.
IdentifiersURN: urn:nbn:se:umu:diva-14003DOI: doi:10.1074/mcp.M300137-MCP200OAI: oai:DiVA.org:umu-14003DiVA: diva2:153674