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Organellar gene transcription and early seedling development are affected in the rpoT;2 mutant of Arabidopsis.
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2004 (English)In: Plant J, ISSN 0960-7412, Vol. 38, no 1, 38-48 p.Article in journal (Refereed) Published
Abstract [en]

An Arabidopsis mutant that exhibited reduced root length was isolated from a population of activation-tagged T-DNA insertion lines in a screen for aberrant root growth. This mutant also exhibited reduced hypocotyl length as well as a delay in greening and altered leaf shape. Molecular genetic analysis of the mutant indicated a single T-DNA insertion in the gene RpoT;2 encoding a homolog of the phage-type RNA polymerase (RNAP), that is targeted to both mitochondria and plastids. A second T-DNA-tagged allele also showed a similar phenotype. The mutation in RpoT;2 affected the light-induced accumulation of several plastid mRNAs and proteins and resulted in a lower photosynthetic efficiency. In contrast to the alterations in the plastid gene expression, no major effect of the rpoT;2 mutation on the accumulation of examined mitochondrial gene transcripts and proteins was observed. The rpoT;2 mutant exhibited tissue-specific alterations in the transcript levels of two other organelle-directed nuclear-encoded RNAPs, RpoT;1 and RpoT;3. This suggests the existence of cross-talk between the regulatory pathways of the three RNAPs through organelle to nucleus communication. These data provide an important information on a role of RpoT;2 in plastid gene expression and early plant development.

Place, publisher, year, edition, pages
2004. Vol. 38, no 1, 38-48 p.
Keyword [en]
Arabidopsis/*genetics/*growth & development/metabolism, Arabidopsis Proteins/metabolism, Base Sequence, DNA Transposable Elements/genetics, DNA; Bacterial/genetics, DNA; Plant/genetics, DNA-Directed RNA Polymerases/genetics, Gene Expression Regulation; Developmental, Gene Expression Regulation; Plant, Genes; Plant, Mitochondria/genetics, Mutagenesis; Insertional, Mutation, Organelles/genetics, Photosystem II Protein Complex/metabolism, Plastids/genetics, Seedling/growth & development
URN: urn:nbn:se:umu:diva-14149DOI: doi:10.1111/j.1365-313X.2004.02022.xPubMedID: 15053758OAI: diva2:153820
Available from: 2007-05-23 Created: 2007-05-23 Last updated: 2015-04-29Bibliographically approved

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Gardeström, Per
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Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)

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