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The association of the antenna system to photosystem I in higher plants. Cooperative interactions stabilize the supramolecular complex and enhance red-shifted spectral forms.
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0002-7906-6891
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2005 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, Vol. 280, no 35, 31050-8 p.Article in journal (Refereed) Published
Abstract [en]

We report on the association of the antenna system to the reaction center in Photosystem I. Biochemical analysis of mutants depleted in antenna polypeptides showed that the binding of the antenna moiety is strongly cooperative. The minimal building block for the antenna system was shown to be a dimer. Specific protein-protein interactions play an important role in antenna association, and the gap pigments, bound at the interface between core and antenna, are proposed to mediate these interactions Gap pigments have been characterized by comparing the spectra of the Photosystem I to those of the isolated antenna and core components. CD spectroscopy showed that they are involved in pigment-pigment interactions, supporting their relevance in energy transfer from antenna to the reaction center. Moreover, gap pigments contribute to the red-shifted emission forms of Photosystem I antenna. When compared with Photosystem II, the association of peripheral antenna complexes in PSI appears to be more stable, but far less flexible and functional implications are discussed.

Place, publisher, year, edition, pages
2005. Vol. 280, no 35, 31050-8 p.
Keyword [en]
Arabidopsis/chemistry/physiology, Arabidopsis Proteins/chemistry/*metabolism, Circular Dichroism, Electrophoresis; Gel; Two-Dimensional, Electrophoresis; Polyacrylamide Gel/methods, Light, Light-Harvesting Protein Complexes/*physiology, Molecular Structure, Photosystem I Protein Complex/*physiology, Pigments; Biological/chemistry, Protein Conformation, Protein Denaturation, Thylakoids/chemistry/metabolism
Identifiers
URN: urn:nbn:se:umu:diva-14213DOI: doi:10.1074/jbc.M502935200PubMedID: 15983042OAI: oai:DiVA.org:umu-14213DiVA: diva2:153884
Available from: 2007-05-24 Created: 2007-05-24 Last updated: 2015-04-29Bibliographically approved

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CiteExportLink to record
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