Change search
ReferencesLink to record
Permanent link

Direct link
Pigment binding, fluorescence properties, and oligomerization behavior of Lhca5, a novel light-harvesting protein.
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0002-7906-6891
2005 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, Vol. 280, no 7, 5163-8 p.Article in journal (Refereed) Published
Abstract [en]

A new potential light-harvesting protein, named Lhca5, was recently detected in higher plants. Because of the low amount of Lhca5 in thylakoid membranes, the isolation of a native Lhca5 pigment-protein complex has not been achieved to date. Therefore, we used in vitro reconstitution to analyze whether Lhca5 binds pigments and is actually an additional light-harvesting protein. By this approach we could demonstrate that Lhca5 binds pigments in a unique stoichiometry. Analyses of pigment requirements for light-harvesting complex formation by Lhca5 revealed that chlorophyll b is the only indispensable pigment. Fluorescence measurements showed that ligated chlorophylls and carotenoids are arranged in a way that allows directed energy transfer within the light-harvesting complex. Reconstitutions of Lhca5 together with other Lhca proteins resulted in the formation of heterodimers with Lhca1. This result demonstrates that Lhca5 is indeed a protein belonging to the light-harvesting antenna of photosystem I. The properties of Lhca5 are compared with those of previously characterized Lhca proteins, and the consequences of an additional Lhca protein for the composition of the light-harvesting antenna of photosystem I are discussed in view of the recently published photosystem I structure of the pea.

Place, publisher, year, edition, pages
2005. Vol. 280, no 7, 5163-8 p.
Keyword [en]
Arabidopsis/*chemistry/metabolism, Arabidopsis Proteins/*chemistry/genetics/*metabolism, Carotenoids/metabolism, Chlorophyll/metabolism, Dimerization, Fluorescence, Light-Harvesting Protein Complexes/*chemistry/genetics/*metabolism, Photosystem I Protein Complex/chemistry/genetics/metabolism, Pigments; Biological/*metabolism, Protein Structure; Quaternary
URN: urn:nbn:se:umu:diva-14224DOI: doi:10.1074/jbc.M411248200PubMedID: 15563470OAI: diva2:153895
Available from: 2007-05-24 Created: 2007-05-24 Last updated: 2015-04-29Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Jansson, Stefan
By organisation
Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 17 hits
ReferencesLink to record
Permanent link

Direct link