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Global analysis of low-molecular-weight compounds in human plasma using GC/TOF-MS
Umeå University, Faculty of Science and Technology, Department of Chemistry. (Computational Life Science Cluster (CLiC))
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
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2004 (English)In: DRUG METABOLISM REVIEWS, ISSN 0360-2532, Vol. 36, 246-246 p.Article in journal (Other academic) Published
Abstract [en]

We developed a method for analysis of low-molecular-weight compounds (LMWC) in human plasma involving extraction of metabolites by organic solvents, derivatization of extract and final analysis by GC/TOF-MS. The overall strategy for the development of the method was based on using design-of-experimental (DOE), and data evaluation based on multivariate statistical tools like PCA and PLS. The results showed that the extraction efficiency for different solvents varied, and that methanol was important for high reproducibility. More than 300 compounds could be detected in one analysis. Forty five of them were identified, including amino acids, lipids and free fatty acids, organic acids, carbohydrates and so on. The quantitative data of these metabolites showed, with two exceptions, high precision and good linearity between response and concentration. By using this method it is now possible to analyze plasma samples with high throughput to identify metabolic biomarkers for different kinds of diseases.

Place, publisher, year, edition, pages
2004. Vol. 36, 246-246 p.
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-14336OAI: oai:DiVA.org:umu-14336DiVA: diva2:154007
Note

Meeting Abstract 492 Suppl. 1, AUG 2004

Available from: 2008-04-17 Created: 2008-04-17 Last updated: 2012-09-05

Open Access in DiVA

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http://www.informaworld.com/smpp/section?content=a754068934&fulltext=713240928

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Trygg, JohanMarklund, Stefan

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