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Phase behaviour of the 1-monooleoyl- rac-glycerol / n-octyl-ß-D-glucoside/water system
Umeå University, Faculty of Science and Technology, Chemistry.
2004 (English)In: Progress in Colloid and Polymer Science, Springer Berlin, Heidelberg , 2004, 36-9 p.Chapter in book (Refereed)
Abstract [en]

Obtaining high-quality crystals for X-ray diffraction from membrane proteins has proven to be a difficult task. One recently presented method utilises the cubic phases formed by 1-monooleoyl-rac-glycerol (MO). Removing the proteins from their native environment requires the use of surfactants. One commonly used surfactant is n-octyl--D-glucopyranoside (OG). Using NMR techniques and visual observations, the ternary phase diagram of MO/OG/2>H2O was outlined at 25 °C. The preliminary data show that all phases present in the binary systems at this temperature are also found in the ternary. Further, at the OG-rich side, an additional phase that appears to be hexagonal occurs. Addition of minor amounts (1.5 wt/wt) of OG converts the cubic phases of MO to a lamellar structure, while the OG-rich cubic phase is able to dissolve about 15 wt/wt MO. OG in water forms a large micellar solution phase. Increasing the MO concentration at constant water content leads to a series of two- and three-phase areas in which one or two phases are in equilibrium with almost pure water.

Place, publisher, year, edition, pages
Springer Berlin, Heidelberg , 2004. 36-9 p.
URN: urn:nbn:se:umu:diva-14420DOI: doi:10.1007/b11618ISBN: 978-3-540-00553-7OAI: diva2:154091
Subject Collection Chemistry and Materials Science Volume 123Available from: 2007-05-31 Created: 2007-05-31Bibliographically approved

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