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Transient Aggregation and Stable Dimerization Induced by Introducing an Alzheimer Sequence into a Water-Soluble Protein
Umeå University, Faculty of Science and Technology, Chemistry.
2004 (English)In: Biochemistry, Vol. 43, no 41, 12964-78 p.Article in journal (Refereed) Published
Abstract [en]

Transient contacts between denatured polypeptide chains are likely to play an important part in the initial stages of protein aggregation and fibrillation. To analyze the nature of such contacts, we have carried out a protein engineering study of the 102-residue protein U1A, which aggregates transiently in the wild-type form during refolding from the guanidinium chloride-denatured state. We have prepared a series of mutants with increased aggregation tendencies by increasing the homology between two -strands of U1A and the Alzheimer peptide (-AP). These mutants undergo transient aggregation during refolding, as measured by concentration dependence, double-jump experiments, and binding of ANS, a probe for exposed hydrophobic patches on protein surfaces. The propensity to aggregate increases with increasing homology to -AP. Further, the degree of transient ANS binding correlates reasonably well with the structural parameters recently shown to play a role in the fibrillation of natively unfolded proteins. Two mutants highly prone to transient aggregation, U1A-J and U1A-G, were also studied by NMR. Secondary structural elements of the U1A-J construct (with lower -AP homology) are very similar to those observed in U1A-wt. In contrast, the high-homology construct U1A-G exhibits local unfolding of the C-terminal helix, which packs against the -sheet in the wild-type protein. U1A-G is mainly dimeric according to 15N spin relaxation data, and the dimer interface most likely involves the -sheet. Our data suggest that the transient aggregate relies on specific intermolecular interactions mediated by structurally flexible regions and that contacts may be formed in different -strand registers.

Place, publisher, year, edition, pages
2004. Vol. 43, no 41, 12964-78 p.
Identifiers
URN: urn:nbn:se:umu:diva-14796DOI: doi:10.1021/bi048509kOAI: oai:DiVA.org:umu-14796DiVA: diva2:154468
Available from: 2008-04-18 Created: 2008-04-18 Last updated: 2011-01-12Bibliographically approved

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