Self-aggregation: an intrinsic property of GM1 in lipid bilayers
2007 (English)In: Molecular membrane biology, ISSN 0968-7688, E-ISSN 1464-5203, Vol. 24, no 2, 102-112 p.Article in journal (Refereed) Published
We demonstrate that the ganglioside GM1 in lipid bilayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) exhibits a non-uniform lateral distribution, i.e., enriched regions of GM1 molecules are formed, which is an argument in favour of self-aggregation of GM1 being an intrinsic property of GM1 ganglioside. This was concluded from energy transfer/migration studies of BODIPY-labelled gangliosides by means of time-resolved fluorescence lifetime and depolarization experiments. Three fluorophore-labelled gangliosides were synthesized to include either of two spectroscopically different BODIPY groups. These were specifically localized either in the polar headgroup region or in the non-polar region of the lipid bilayer. An eventual ganglioside-ganglioside affinity/aggregation induced by the BODIPY groups was experimentally excluded, which suggests their use in examining the influence of GM1 in more complex systems.
Place, publisher, year, edition, pages
London: Taylor & Francis , 2007. Vol. 24, no 2, 102-112 p.
Ganglioside GM1, lipid rafts, aggregation, DDEM, DAET, FRET
IdentifiersURN: urn:nbn:se:umu:diva-15005DOI: doi:10.1080/09687860600995235OAI: oai:DiVA.org:umu-15005DiVA: diva2:154677