Germ line insertions of moloney murine leukemia virus in the TLL mouse causes site-specific differences in lymphoma/leukemia frequency and tumor immunophenotype
2006 (English)In: Anticancer Research, ISSN 0250-7005, Vol. 26, no 4B, 2873-2878 p.Article in journal (Refereed) Published
Background: Moloney murine leukemia virus (Mo-MLV) has proven valuable for studies of the pathogenesis of malignant lymphoma. Inoculation of newborn mice induces T cell lymphoma with 100% incidence. The TLL (T cell lymphoma/leukemia)-strain was previously established and was shown to spontaneously develop T cell lymphoma at high frequency.
Materials and Methods: Differential screening of cDNA libraries was performed to discover an involvement of Mo-MLV and genomic sequencing was used to identify the chromosomal position of Mo-MLV proviral integration sites. Immunophenotypes of the tumors were established by flow cytometry. Disease frequency curves were created according to the Kaplan-Meier method.
Results: Two independent Mo-MLV germ line integrations were characterized on chromosomes 2 and 14, giving rise to two substrains of mice denoted TLL-2 and TLL-14. The chromosomal position of the integrated provirus affected the frequency of disease, as well as the immunophenotype of the tumors.
Conclusion: The data suggest that factors influencing the transcriptional activity of the chromosomal regions, leading to differences in proviral expression, could underlie the observed difference in tumor frequency.
Place, publisher, year, edition, pages
2006. Vol. 26, no 4B, 2873-2878 p.
Animals, CD4-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/immunology, Immunophenotyping, Leukemia; T-Cell/*genetics/immunology/*virology, Lymphoma; T-Cell/*genetics/immunology/*virology, Mice, Mice; Inbred C57BL, Moloney murine leukemia virus/*genetics, Virus Integration
Cancer and Oncology
Research subject Oncology
IdentifiersURN: urn:nbn:se:umu:diva-15295PubMedID: 16886607OAI: oai:DiVA.org:umu-15295DiVA: diva2:154967