Change search
ReferencesLink to record
Permanent link

Direct link
Characterization of the photosynthetic apparatus in cortical bark chlorenchyma of Scots pine.
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Show others and affiliations
2006 (English)In: Planta, ISSN 0032-0935, Vol. 223, no 6, 1165-77 p.Article in journal (Refereed) Published
Abstract [en]

Winter-induced inhibition of photosynthesis in Scots pine (Pinus sylvestris L.) needles is accompanied by a 65% reduction of the maximum photochemical efficiency of photosystem II (PSII), measured as F v/F m, but relatively stable photosystem I (PSI) activity. In contrast, the photochemical efficiency of PSII in bark chlorenchyma of Scots pine twigs was shown to be well preserved, while PSI capacity was severely decreased. Low-temperature (77 K) chlorophyll fluorescence measurements also revealed lower relative fluorescence intensity emitted from PSI in bark chlorenchyma compared to needles regardless of the growing season. Nondenaturating SDS-PAGE analysis of the chlorophyll–protein complexes also revealed much lower abundance of LHCI and the CPI band related to light harvesting and the core complex of PSI, respectively, in bark chlorenchyma. These changes were associated with a 38% reduction in the total amount of chlorophyll in the bark chlorenchyma relative to winter needles, but the Chl a/b ratio and carotenoid composition were similar in the two tissues. As distinct from winter pine needles exhibiting ATP/ADP ratio of 11.3, the total adenylate content in winter bark chlorenchyma was 2.5-fold higher and the estimated ATP/ADP ratio was 20.7. The photochemical efficiency of PSII in needles attached to the twig recovered significantly faster (28–30 h) then in detached needles. Fluorescence quenching analysis revealed a high reduction state of Q A and the PQ-pool in the green bark tissue. The role of bark chlorenchyma and its photochemical performance during the recovery of photosynthesis from winter stress in Scots pine is discussed.

Place, publisher, year, edition, pages
2006. Vol. 223, no 6, 1165-77 p.
Keyword [en]
Adenosine Diphosphate/metabolism, Adenosine Triphosphate/metabolism, Chlorophyll/metabolism/physiology, Chromatography; High Pressure Liquid, Fluorescence, Malate Dehydrogenase/metabolism, Oxidation-Reduction, Peptides/metabolism, Phosphoenolpyruvate Carboxylase/metabolism, Photosynthesis/*physiology, Photosystem II Protein Complex/*chemistry/physiology, Pigments; Biological/analysis, Pinus sylvestris/anatomy & histology/*metabolism/physiology, Plant Bark/anatomy & histology/*metabolism/physiology, Seasons, Temperature
URN: urn:nbn:se:umu:diva-15787DOI: doi:10.1007/s00425-005-0164-1PubMedID: 16333639OAI: diva2:155459
Available from: 2007-08-03 Created: 2007-08-03 Last updated: 2015-04-29Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Malmberg, GunillaGardeström, PerHurry, VaughanÖquist, Gunnar
By organisation
Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 38 hits
ReferencesLink to record
Permanent link

Direct link