Occurrence and characterisation of the hydrogen-evolving enzyme in Frankia sp.
2006 (English)In: International journal of hydrogen energy, ISSN 0360-3199, Vol. 31, no 11, 1445-1451 p.Article in journal (Refereed) Published
An increase in hydrogen evolution from the hydrogen-evolving enzyme in the actinomycete Frankia was recorded in the presence of nickel. Immunogold localisation analysis of the intracellular distribution of hydrogenase proteins indicated that they were evenly distributed in the membranes and cytosol of both hyphae and vesicles. In addition, molecular characterisation of the hydrogen-evolving enzyme at the proteomic level, using two-dimensional gel electrophoresis combined with mass spectrometry, confirmed that the Frankia hydrogen-evolving enzyme is similar to the cyanobacterial bidirectional hydrogenase of Anabena siamensis.
Place, publisher, year, edition, pages
Oxford: Pergamon , 2006. Vol. 31, no 11, 1445-1451 p.
Frankia; Hydrogen; Hydrogen-evolving hydrogenase; Nickel
IdentifiersURN: urn:nbn:se:umu:diva-15951DOI: 10.1016/j.ijhydene.2006.06.009OAI: oai:DiVA.org:umu-15951DiVA: diva2:155623