Side-chain and backbone amide bond requirements for glycopeptide stimulation of T-cells obtained in a mouse model for rheumatoid arthritis
2006 (English)In: Bioorganic & Medicinal Chemistry, ISSN 0968-0896, E-ISSN 1464-3391, Vol. 14, no 17, 5921-5932 p.Article in journal (Refereed) Published
Collagen induced arthritis (CIA) is the most studied animal model for rheumatoid arthritis and is associated with the MHC class II molecule Aq. T-cell recognition of a peptide from type II collagen, CII256–270, bound to Aq is a requirement for development of CIA. Lysine 264 is the major T-cell recognition site of CII256–270 and CIA is in particular associated with recognition of lysine 264 after posttranslational hydroxylation and subsequent attachment of a β-d-galactopyranosyl moiety. In this paper we have studied the structural requirements of collagenous glycopeptides required for T-cell stimulation, as an extension of earlier studies of the recognition of the galactose moiety. Synthesis and evaluation of alanine substituted glycopeptides revealed that there are T-cells that only recognise the galactosylated hydroxylysine 264, and no other amino acid side chains in the peptide. Other T-cells also require glutamic acid 266 as a T-cell contact point. Introduction of a methylene ether isostere instead of the amide bond between residues 260 and 261 allowed weaker recognition by some, but not all, of the T-cells. Altogether, these results allowed us to propose a model for glycopeptide recognition by the T-cells, where recognition from one or the other side of the galactose moiety could explain the different binding patterns of the T-cells.
Place, publisher, year, edition, pages
Oxford: Pergamon Press , 2006. Vol. 14, no 17, 5921-5932 p.
Glycopeptide; Collagen, T-cell, Rheumatoid arthritis, Amide bond isostere, Peptide mimetics
IdentifiersURN: urn:nbn:se:umu:diva-16105DOI: doi:10.1016/j.bmc.2006.05.023OAI: oai:DiVA.org:umu-16105DiVA: diva2:155778