Fyn binding protein, Fyb, interacts with mammalian actin binding protein, mAbp1.
2005 (English)In: FEBS Letters, ISSN 0014-5793, Vol. 579, no 11, 2339-2347 p.Article in journal (Refereed) Published
The immune cell specific protein Fyn-T binding protein (Fyb) has been identified as a target of the Yersinia antiphagocytic effector Yersinia outer protein H (YopH), but its role in macrophages is unknown. By using Fyb domains as bait to screen a mouse lymphoma cDNA library, we identified a novel interaction partner, mammalian actin binding protein 1 (mAbp1). We show that mAbp1 binds the Fyb N-terminal via its C-terminally located src homology 3 domain. The interaction between Fyb and mAbp1 is detected in macrophage lysates and the proteins co-localize with F-actin in the leading edge. Hence, mAbp1 is likely to constitute a downstream effector of Fyb involved in F-actin dynamics.
Place, publisher, year, edition, pages
2005. Vol. 579, no 11, 2339-2347 p.
Actins/metabolism, Adaptor Proteins; Signal Transducing/chemistry/genetics/*metabolism, Amino Acid Motifs, Animals, Bacterial Outer Membrane Proteins/metabolism, Cell Line, Mice, Microfilament Proteins/genetics/*metabolism, Phosphorylation, Phosphotyrosine/metabolism, Protein Binding, Protein Transport, Protein-Tyrosine-Phosphatase/metabolism, Proto-Oncogene Proteins/metabolism, Proto-Oncogene Proteins c-fyn, Two-Hybrid System Techniques, src Homology Domains/genetics, src-Family Kinases/metabolism
IdentifiersURN: urn:nbn:se:umu:diva-16652DOI: 10.1016/j.febslet.2005.03.031PubMedID: 15848169OAI: oai:DiVA.org:umu-16652DiVA: diva2:156325