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Mnt loss triggers Myc transcription targets, proliferation, apoptosis, and transformation.
Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology). (Nilsson)
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2004 (English)In: Mol Cell Biol, ISSN 0270-7306, Vol. 24, no 4, 1560-9 p.Article in journal (Refereed) Published
Abstract [en]

Myc oncoproteins are overexpressed in most cancers and are sufficient to accelerate cell proliferation and provoke transformation. However, in normal cells Myc also triggers apoptosis. All of the effects of Myc require its function as a transcription factor that dimerizes with Max. This complex induces genes containing CACGTG E-boxes, such as Ornithine decarboxylase (Odc), which harbors two of these elements. Here we report that in quiescent cells the Odc E-boxes are occupied by Max and Mnt, a putative Myc antagonist, and that this complex is displaced by Myc-Max complexes in proliferating cells. Knockdown of Mnt expression by stable retroviral RNA interference triggers many targets typical of the "Myc" response and provokes accelerated proliferation and apoptosis. Strikingly, these effects of Mnt knockdown are even manifest in cells lacking c-myc. Moreover, Mnt knockdown is sufficient to transform primary fibroblasts in conjunction with Ras. Therefore, Mnt behaves as a tumor suppressor. These findings support a model where Mnt represses Myc target genes and Myc functions as an oncogene by relieving Mnt-mediated repression.

Place, publisher, year, edition, pages
2004. Vol. 24, no 4, 1560-9 p.
Keyword [en]
Animals, Apoptosis, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Cell Division, Cell Transformation; Neoplastic, Cells; Cultured, Fibroblasts, Gene Expression Regulation; Neoplastic, Genes; Suppressor, Mice, Mice; Inbred BALB C, Nuclear Proteins/*deficiency/genetics/metabolism, Ornithine Decarboxylase/genetics, Phenotype, Proto-Oncogene Proteins c-myc/*metabolism, RNA; Messenger/genetics/metabolism, Repressor Proteins, Transcription; Genetic/*genetics
URN: urn:nbn:se:umu:diva-16709PubMedID: 14749372OAI: diva2:156382
Available from: 2007-10-09 Created: 2007-10-09 Last updated: 2011-01-12Bibliographically approved

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