Diet-dependent depletion of queuosine in tRNAs in Caenorhabditis elegans does not lead to a developmental block.
2007 (English)In: J Biosci, ISSN 0250-5991, Vol. 32, no 4, 747-54 p.Article in journal (Refereed) Published
Queuosine (Q), a hypermodified nucleoside,occurs at the wobble position of transfer RNAs (tRNAs)with GUN anticodons. In eubacteria, absence of Q affects messenger RNA (mRNA) translation and reduces the virulence of certain pathogenic strains. In animal cells,changes in the abundance of Q have been shown to correlate with diverse phenomena including stress tolerance, cell proliferation and tumour growth but the function of Q in animals is poorly understood. Animals are thought to obtain Q (or its analogues) as a micronutrient from dietary sources such as gut micro flora. However,the difficulty of maintaining animals under bacteria-free conditions on Q-deficient diets has severely hampered the study of Q metabolism and function in animals. In this study,we show that as in higher animals, tRNAs in the nematode Caenorhabditis elegans are modified by Q and its sugar derivatives. When the worms were fed on Q-deficient Escherichia coli, Q modification was absent from the worm tRNAs suggesting that C.elegans lacks a de novo pathway of Q biosynthesis. The inherent advantages of C.elegans as a model organism, and the simplicity of conferring a Q-deficient phenotype on it make it an ideal system to investigate the function of Q modification in tRNA.
Place, publisher, year, edition, pages
2007. Vol. 32, no 4, 747-54 p.
IdentifiersURN: urn:nbn:se:umu:diva-16728PubMedID: 17762147OAI: oai:DiVA.org:umu-16728DiVA: diva2:156401