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Diet-dependent depletion of queuosine in tRNAs in Caenorhabditis elegans does not lead to a developmental block.
Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology). (Björk)
Umeå Centre for Molecular Pathogenesis (UCMP).
2007 (English)In: J Biosci, ISSN 0250-5991, Vol. 32, no 4, 747-54 p.Article in journal (Refereed) Published
Abstract [en]

Queuosine (Q), a hypermodified nucleoside,occurs at the wobble position of transfer RNAs (tRNAs)with GUN anticodons. In eubacteria, absence of Q affects messenger RNA (mRNA) translation and reduces the virulence of certain pathogenic strains. In animal cells,changes in the abundance of Q have been shown to correlate with diverse phenomena including stress tolerance, cell proliferation and tumour growth but the function of Q in animals is poorly understood. Animals are thought to obtain Q (or its analogues) as a micronutrient from dietary sources such as gut micro flora. However,the difficulty of maintaining animals under bacteria-free conditions on Q-deficient diets has severely hampered the study of Q metabolism and function in animals. In this study,we show that as in higher animals, tRNAs in the nematode Caenorhabditis elegans are modified by Q and its sugar derivatives. When the worms were fed on Q-deficient Escherichia coli, Q modification was absent from the worm tRNAs suggesting that C.elegans lacks a de novo pathway of Q biosynthesis. The inherent advantages of C.elegans as a model organism, and the simplicity of conferring a Q-deficient phenotype on it make it an ideal system to investigate the function of Q modification in tRNA.

Place, publisher, year, edition, pages
2007. Vol. 32, no 4, 747-54 p.
URN: urn:nbn:se:umu:diva-16728PubMedID: 17762147OAI: diva2:156401
Available from: 2008-12-14 Created: 2008-12-14 Last updated: 2011-01-11Bibliographically approved

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Gaur, RahulBjörk, GlennTuck, Simon
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Molecular Biology (Faculty of Science and Technology)Umeå Centre for Molecular Pathogenesis (UCMP)

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