The Kluyveromyces lactis γ-toxin targets tRNA anticodons.
2005 (English)In: RNA: A publication of the RNA Society, ISSN 1355-8382, Vol. 11, no 11, 1648-1654 p.Article in journal (Refereed) Published
Kluyveromyces lactis killer strains secrete a heterotrimeric toxin (zymocin), which causes an irreversible growth arrest of sensitive yeast cells. Despite many efforts, the target(s) of the cytotoxic gamma-subunit of zymocin has remained elusive. Here we show that three tRNA species tRNA(Glu)(mcm(5)s(2)UUC), tRNA(Lys)(mcm(5)s(2)UUU), and tRNA(Gln)(mcm(5)s(2)UUG) are the targets of gamma-toxin. The toxin inhibits growth by cleaving these tRNAs at the 3' side of the modified wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U). Transfer RNA lacking a part of or the entire mcm(5) group is inefficiently cleaved by gamma-toxin, explaining the gamma-toxin resistance of the modification-deficient trm9, elp1-elp6, and kti11-kti13 mutants. The K. lactis gamma-toxin is the first eukaryotic toxin shown to target tRNA.
Place, publisher, year, edition, pages
2005. Vol. 11, no 11, 1648-1654 p.
Anticodon/*drug effects, Drug Resistance; Fungal/genetics, Gene Expression, Kluyveromyces/*chemistry, Mutation/genetics, Mycotoxins/genetics/*pharmacology, Nucleic Acid Conformation, RNA; Transfer/*drug effects, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/physiology
IdentifiersURN: urn:nbn:se:umu:diva-16758DOI: 10.1261/rna.2172105PubMedID: 16244131OAI: oai:DiVA.org:umu-16758DiVA: diva2:156431