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Bacterial oxidation of ferrous iron at low temperatures.
Institute of Geotechnics, Slovak Academy of Sciences, Watsonova 45, SK-043 53 Koice, Slovakia.
Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology). (Dopson)
Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology). (Dopson)
Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology). (Lindström)
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2007 (English)In: Biotechnology and Bioengineering, ISSN 0006-3592, Vol. 97, no 6, 1470-1478 p.Article in journal (Refereed) Published
Abstract [en]

This study comprises the first report of ferrous iron oxidation by psychrotolerant, acidophilic iron-oxidizing bacteria capable of growing at 5 degrees C. Samples of mine drainage-impacted surface soils and sediments from the Norilsk mining region (Taimyr, Siberia) and Kristineberg (Skellefte district, Sweden) were inoculated into acidic ferrous sulfate media and incubated at 5 degrees C. Iron oxidation was preceded by an approximately 3-month lag period that was reduced in subsequent cultures. Three enrichment cultures were chosen for further work and one culture designated as isolate SS3 was purified by colony isolation from a Norilsk enrichment culture for determining the kinetics of iron oxidation. The 16S rRNA based phylogeny of SS3 and two other psychrotolerant cultures, SS5 from Norilsk and SK5 from Northern Sweden, was determined. Comparative analysis of amplified 16S rRNA gene sequences showed that the psychrotolerant cultures aligned within Acidithiobacillus ferrooxidans. The rate constant of iron oxidation by growing cultures of SS3 was in the range of 0.0162-0.0104 h(-1) depending on the initial pH. The oxidation kinetics followed an exponential pattern, consistent with a first order rate expression. Parallel iron oxidation by a mesophilic reference culture of Acidithiobacillus ferrooxidans was extremely slow and linear. Precipitates harvested from the 5 degrees C culture were identified by X-ray diffraction as mixtures of schwertmannite (ideal formula Fe(8)O(8)(OH)(6)SO(4)) and jarosite (KFe(3)(SO(4))(2)(OH)(6)). Jarosite was much more dominant in precipitates produced at 30 degrees C. (c) 2007 Wiley Periodicals, Inc.

Place, publisher, year, edition, pages
2007. Vol. 97, no 6, 1470-1478 p.
Keyword [en]
Acidithiobacillus/*classification/genetics/isolation & purification/*metabolism, Iron/*metabolism, Oxidation-Reduction, Temperature
URN: urn:nbn:se:umu:diva-16764DOI: 10.1002/bit.21371PubMedID: 17304566OAI: diva2:156437
Available from: 2007-10-10 Created: 2007-10-10Bibliographically approved
In thesis
1. Physiology and Genetics of Acidithiobacillus species: Applications for Biomining
Open this publication in new window or tab >>Physiology and Genetics of Acidithiobacillus species: Applications for Biomining
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Bacteria from the genus Acidithiobacillus are often associated with biominingand acid mine drainage. Biomining utilises acidophilic, sulphur and ironoxidising microorganisms for recovery of metals from sulphidic low grade oresand concentrates. Acid mine drainage results in acidification and contaminationwith metals of soil and water emanating from the dissolution of metal sulphidesfrom deposits and mine waste storage. Acidophilic microorganisms play acentral role in these processes by catalysing aerobic oxidation of sulphides.Acceleration of mineral solubilisation is a positive aspect in biomining whereas,in acid mine drainage it is undesirable and accordingly, microbial iron andsulphur oxidation is promoted in the first case and measures are taken to inhibitit in the second case. In this thesis, several approaches were taken in order tounderstand and increase oxidation efficiency in biomining and to gain an insightinto the biochemical reactions taking place in these environments. A laboratoryscale bioreactor was designed and tested allowing simulation of bioleaching inheaps of mine tailings at different aeration, irrigation and particle size conditions(Paper I). A new psychrotolerant strain of Acidithiobacillus ferrooxidans wascharacterised that has an application in boreal heap bioleaching. Iron, reducedinorganic sulphur compound oxidation and bioleaching of various ores by thisstrain was studied as well as gene expression during oxidation of tetrathionateand/or ferrous iron (Papers III & IV). Expression and regulation of atetrathionate hydrolase from Acidithiobacillus caldus, a key enzyme in reducedinorganic sulphur compound metabolism of this bacterium was investigated andthe presence of this enzyme in a bioleaching mixed culture was shown. The genecluster that harbours the gene coding for tetrathionate hydrolase (tetH) wasdescribed for the first time (Paper II).

Place, publisher, year, edition, pages
Umeå: Molekylärbiologi (Teknisk-naturvetenskaplig fakultet), 2008. 73 p.
National Category
urn:nbn:se:umu:diva-1545 (URN)978-91-7264-509-7 (ISBN)
Public defence
2008-03-07, Major Grove, 6L, Umeå University, Dept. of Molecular Biology, 10:00 (English)
Available from: 2008-02-15 Created: 2008-02-15 Last updated: 2009-06-30Bibliographically approved

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Rzhepishevska, OlenaDopson, MarkLindström, Börje
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