Lateral Diffusion of Cholesterol and Dimyristoylphosphatidylcholine in a Lipid Bilayer Measured by Pulsed Field Gradient NMR Spectroscopy
2002 (English)In: Biophys Journal, Vol. 83, no 5, 2702-4 p.Article in journal (Refereed) Published
The pulsed field gradient NMR method for measuring self-diffusion has been used for a direct determination of the lateral diffusion coefficient of cholesterol, fluorine labeled at the 6-position, for an oriented lamellar liquid-crystalline phase of dimyristoylphosphatidylcholine (DMPC)/cholesterol/water. It is found that the diffusion coefficients of DMPC and cholesterol are equal over a large temperature interval. The apparent energy of activation for the diffusion process (58 kJ/mol) is about the same as for a lamellar phase of DMPC/water, whereas the phospholipid lateral diffusion coefficient is approximately four times smaller in the presence of cholesterol.
Place, publisher, year, edition, pages
2002. Vol. 83, no 5, 2702-4 p.
IdentifiersURN: urn:nbn:se:umu:diva-16779OAI: oai:DiVA.org:umu-16779DiVA: diva2:156452