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First use of two-dimensional polyacrylamide gel electrophoresis to determine phylogenetic relationships.
Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology).
2004 (English)In: J Microbiol Methods, ISSN 0167-7012, Vol. 58, no 3, 297-302 p.Article in journal (Refereed) Published
Abstract [en]

Methods for microbial classification are not always capable of distinguishing between isolates at the species level. We have previously characterised four Ferroplasma isolates that were >98.9% similar at the 16S rDNA level, the isolates showed marked phenotypic differences, and one isolate was borderline on the 70% species boundary from DNA-DNA similarity data. In this study we have used statistical comparisons of two-dimensional polyacylamide gel electrophoresis gels for classification of closely related isolates. From the protein profile similarities an un-rooted tree was constructed that was congruent with a tree derived from DNA-DNA similarities.

Place, publisher, year, edition, pages
2004. Vol. 58, no 3, 297-302 p.
Keyword [en]
Archaea/classification/*genetics, Archaeal Proteins/*genetics, Electrophoresis; Gel; Two-Dimensional/*methods, Phylogeny
URN: urn:nbn:se:umu:diva-16792PubMedID: 15279933OAI: diva2:156465
Available from: 2007-10-12 Created: 2007-10-12 Last updated: 2011-01-12Bibliographically approved

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Dopson, Mark
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Molecular Biology (Faculty of Science and Technology)

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