The DrosDel deletion collection: A Drosophila genomewide chromosomal deficiency resource
2007 (English)In: Genetics, ISSN 0016-6731, E-ISSN 1943-2631, Vol. 177, no 1, 615-629 p.Article in journal (Refereed) Published
We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitatesselection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of other mapped FRT-bearing elements that, when combined with the DrosDel collection, can theoretically generate over half a million precisely mapped deletions.
Place, publisher, year, edition, pages
Austin, Tex.: Genetics Society of America , 2007. Vol. 177, no 1, 615-629 p.
IdentifiersURN: urn:nbn:se:umu:diva-16815DOI: 10.1534/genetics.107.076216PubMedID: 17720900OAI: oai:DiVA.org:umu-16815DiVA: diva2:156488