Thioredoxin-2 affects lifespan and oxidative stress in Drosophila
2007 (English)In: Hereditas, ISSN 0018-0661, E-ISSN 1601-5223, Vol. 144, no 1, 25-32 p.Article in journal (Other academic) Published
Thioredoxins are proteins that have thiol-reducing activity and a characteristic conserved active site (WCGPC). They have
several documented functions, e.g. roles in defences against oxidative stress and as electron donors for ribonucleotidereductase.
In Drosophila melanogaster there are three ‘‘classical’’ thioredoxins with the conserved active site: deadhead,
ThioredoxinT and Thioredoxin-2. Here, we report the creation of null-mutations in the Thioredoxin-2 (Trx-2) gene.
Characterization of two Trx-2 mutants indicated that Trx-2 affects the lifespan of D. melanogaster, and is involved in the
organism’s oxidative stress protection system. We found that the mutants have a shorter lifespan than wild-type flies, and
thioredoxin double mutant flies showed lower tolerance to oxidative stress than wild-type flies, while flies carrying multiple
copies of a Trx-2 rescue construct showed higher tolerance. These findings suggest that Trx-2 has modest or redundant
functions in Drosophila physiology under unstressed conditions, but could be important during times of environmental
Place, publisher, year, edition, pages
Oxford: Blackwell , 2007. Vol. 144, no 1, 25-32 p.
Analysis of Variance, Animals, Animals; Genetically Modified, Blotting; Western, Crosses; Genetic, Drosophila/*genetics, Female, Gene Deletion, Genes; Insect, Homozygote, Longevity/*genetics, Male, Membrane Proteins/*genetics/*physiology, Oxidative Stress/*genetics, Thioredoxin/*genetics
IdentifiersURN: urn:nbn:se:umu:diva-17027DOI: 10.1111/j.2007.0018-0661.01990.xPubMedID: 17567437OAI: oai:DiVA.org:umu-17027DiVA: diva2:156700