The ThioredoxinT and deadhead gene pair encode testis- and ovary-specific thioredoxins in Drosophila melanogaster.
2003 (English)In: Chromosoma, ISSN 0009-5915, Vol. 112, no 3, 133-43 p.Article in journal (Other academic) Published
So far, two thioredoxin proteins, DHD and Trx-2, have been biochemically characterized in Drosophila melanogaster. Here, with the cloning and characterization of TrxT we describe an additional thioredoxin with testis-specific expression. TrxT and dhd are arranged as a gene pair, transcribed in opposite directions and sharing a 471 bp regulatory region. We show that this regulatory region is sufficient for correct expression of the two genes. This gene pair makes a good model for unraveling how closely spaced promoters are differentially regulated by a short common control region. Both TrxT and DHD proteins are localized within the nuclei in testes and ovaries, respectively. Use of a transgenic construct expressing TrxT fused to Enhanced Yellow Fluorescent Protein reveals a clear association of TrxT with the Y chromosome lampbrush loops ks-1 and kl-5 in primary spermatocytes. The association is lost in the absence of the Y chromosome. Our results suggest that nuclear thioredoxins may have regulatory functions in the germline.
Place, publisher, year, edition, pages
2003. Vol. 112, no 3, 133-43 p.
Amino Acid Sequence, Animals, Bacterial Proteins, DNA; Complementary/genetics, Drosophila Proteins/*genetics/metabolism, Drosophila melanogaster/*genetics/metabolism, Female, Fluorescent Antibody Technique, Gene Components, Gene Expression, In Situ Hybridization, Luminescent Proteins, Male, Membrane Proteins, Molecular Sequence Data, Ovary/metabolism, Regulatory Sequences; Nucleic Acid/genetics, Sequence Alignment, Sequence Analysis; DNA, Testis/metabolism, Thioredoxin/*genetics/metabolism, Transgenes/genetics, Y Chromosome/metabolism
IdentifiersURN: urn:nbn:se:umu:diva-17037PubMedID: 14579129OAI: oai:DiVA.org:umu-17037DiVA: diva2:156710