Change search
ReferencesLink to record
Permanent link

Direct link
NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila.
Show others and affiliations
2003 (English)In: Genes Dev, ISSN 0890-9369, Vol. 17, no 11, 1402-14 p.Article in journal (Other academic) Published
Abstract [en]

NELF and DSIF collaborate to inhibit elongation by RNA polymerase IIa in extracts from human cells. A multifaceted approach was taken to investigate the potential role of these factors in promoter proximal pausing on the hsp70 gene in Drosophila. Immunodepletion of DSIF from a Drosophila nuclear extract reduced the level of polymerase that paused in the promoter proximal region of hsp70. Depletion of one NELF subunit in salivary glands using RNA interference also reduced the level of paused polymerase. In vivo protein-DNA cross-linking showed that NELF and DSIF associate with the promoter region before heat shock. Immunofluorescence analysis of polytene chromosomes corroborated the cross-linking result and showed that NELF, DSIF, and RNA polymerase IIa colocalize at the hsp70 genes, small heat shock genes, and many other chromosomal locations. Finally, following heat shock induction, DSIF and polymerase but not NELF were strongly recruited to chromosomal puffs harboring the hsp70 genes. We propose that NELF and DSIF cause polymerase to pause in the promoter proximal region of hsp70. The transcriptional activator, HSF, might cause NELF to dissociate from the elongation complex. DSIF continues to associate with the elongation complex and could serve a positive role in elongation

Place, publisher, year, edition, pages
2003. Vol. 17, no 11, 1402-14 p.
Keyword [en]
Animals, Base Sequence, Cell Nucleus/genetics/physiology, Chromosome Mapping, Cloning; Molecular, DNA Primers, DNA-Directed RNA Polymerases/genetics, Drosophila Proteins/*genetics/metabolism, Drosophila melanogaster/embryology/*genetics, Embryo; Nonmammalian/physiology, Gene Deletion, HSP70 Heat-Shock Proteins/*genetics, Heat-Shock Proteins/genetics, Humans, Nuclear Proteins/*genetics/metabolism, Protein Subunits/genetics, RNA Polymerase II/genetics/metabolism, RNA-Binding Proteins/*genetics/metabolism, Repressor Proteins, Salivary Glands/enzymology/physiology, Transcription Factors/*genetics/metabolism
URN: urn:nbn:se:umu:diva-17038PubMedID: 12782658OAI: diva2:156711
Available from: 2007-10-26 Created: 2007-10-26 Last updated: 2011-01-13Bibliographically approved

Open Access in DiVA

No full text

Other links


Search in DiVA

By author/editor
Larsson, Jan
By organisation
Umeå Centre for Molecular Pathogenesis (UCMP) (Faculty of Science and Technology)

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 34 hits
ReferencesLink to record
Permanent link

Direct link