umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Reciprocal regulation of Rac1 and Rho1 in Drosophila circulating immune surveillance cells.
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Pathogenesis (UCMP) (Faculty of Medicine). (Dan Hultmark)
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Pathogenesis (UCMP) (Faculty of Medicine). (Dan Hultmark)
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Pathogenesis (UCMP) (Faculty of Medicine). (Dan Hultmark)
2007 (English)In: J Cell Sci, ISSN 0021-9533, Vol. 120, no Pt 3, 502-11 p.Article in journal (Refereed) Published
Abstract [en]

In many cell types it is evident that the small GTPases Rac and Rho regulate each other's activities. What is unclear is exactly how this regulation occurs. To further elucidate this interaction we examined the activities of Rac1 and Rho1 in Drosophila cellular immune surveillance cells. In larvae the cellular immune response involves circulating cells (hemocytes) that can be recruited from a hematopoietic organ located behind the brain, as well as a sessile population found just underneath the larval cuticle. We demonstrate for the first time that Rho-kinase activation requires both Rho1 and the Drosophila c-Jun N-terminal kinase (Basket). We also show that Rac1, via Basket, regulates Rho1 activity, possibly by inhibiting RhoGAPp190. In the reciprocal pathway, co-expression of dominant negative Rho-kinase and constitutive active Rho1 induces a Rac1-like phenotype. This induction requires the formin Diaphanous. Co-expression of dominant negative Rho-kinase and constitutive active Rho1 also induces filopodia formation, with Diaphanous enriched at the tips. The Rac1-like phenotypes, and filopodia formation, could be blocked by co-expression of dominant negative Rac1. Finally, though dominant negative Rac1 is able to block filopodia formation in the overexpression experiments, only Rac2 is necessary for filopodia formed by hemocytes after parasitization.

Place, publisher, year, edition, pages
2007. Vol. 120, no Pt 3, 502-11 p.
Keyword [en]
Animals, Drosophila/enzymology/genetics/*immunology, Drosophila Proteins/genetics/*immunology/metabolism, Fluorescent Antibody Technique; Direct, Gene Expression Regulation; Enzymologic/*immunology, Hemocytes/cytology/enzymology/*immunology, Immunity; Cellular/immunology, JNK Mitogen-Activated Protein Kinases/immunology/metabolism, Larva, rac GTP-Binding Proteins/genetics/*immunology/metabolism, rho GTP-Binding Proteins/genetics/*immunology/metabolism
Identifiers
URN: urn:nbn:se:umu:diva-17043PubMedID: 17227793OAI: oai:DiVA.org:umu-17043DiVA: diva2:156716
Available from: 2007-10-28 Created: 2007-10-28 Last updated: 2011-01-11Bibliographically approved

Open Access in DiVA

No full text

Other links

PubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=17227793&dopt=Citation

Authority records BETA

Williams, MichaelHabayeb, MazenHultmark, Dan

Search in DiVA

By author/editor
Williams, MichaelHabayeb, MazenHultmark, Dan
By organisation
Umeå Centre for Molecular Pathogenesis (UCMP) (Faculty of Medicine)

Search outside of DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric score

pubmed
urn-nbn
Total: 51 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf