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Expression pattern of Filamin-240 in Drosophila blood cells.
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2006 (English)In: Gene Expr Patterns, ISSN 1567-133X, Vol. 6, no 8, 928-34 p.Article in journal (Refereed) Published
Abstract [en]

The expression pattern of Filamin-240 was studied in subsets of Drosophila blood cells by means of immunofluorescent staining and Western blot analysis with use of an antibody specific to a "filamin-folding domain", a consensus motif profile generated from the 20 existing filamin repeats. Expression of Filamin-240 is restricted to lamellocytes - a special blood cell type of the cellular immune response - and is involved in the regulation of lamellocyte development. In the cher1 homozygous larvae, which lack Filamin-240 protein, a vigorous lamellocyte differentiation occurs which is further enhanced upon in vivo immune challenge by a parasitic wasp, Leptopilina boulardi. By introducing a full-length transgene encoding the Drosophila Filamin-240 protein into the cher1 Filamin-deficient homozygous mutant, the mutant blood cell phenotype was rescued. These data demonstrate that the expression of Filamin-240 is strictly lamellocyte specific in Drosophila blood cells and that the protein is a suppressor of lamellocyte development.

Place, publisher, year, edition, pages
2006. Vol. 6, no 8, 928-34 p.
Keyword [en]
Animals, Animals; Genetically Modified, Blood Cells/cytology/*metabolism/parasitology, Cell Differentiation/immunology, Contractile Proteins/genetics/*metabolism/physiology, DNA; Complementary/isolation & purification, Drosophila/growth & development/*metabolism/parasitology, Gene Expression Profiling, Hemocytes/cytology/metabolism, Immune System/cytology/metabolism, Larva/cytology/metabolism/parasitology, Microfilament Proteins/genetics/*metabolism/physiology, Protein Binding, Protein Isoforms, Tissue Distribution, Wasps/immunology
URN: urn:nbn:se:umu:diva-17050PubMedID: 16616709OAI: diva2:156723
Available from: 2007-10-28 Created: 2007-10-28 Last updated: 2011-01-11Bibliographically approved

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Hultmark, Dan
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Umeå Centre for Molecular Pathogenesis (UCMP) (Faculty of Medicine)

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