umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Drosophila Imd pathway.
Visa övriga samt affilieringar
2005 (Engelska)Ingår i: EMBO J, ISSN 0261-4189, Vol. 24, nr 19, s. 3423-34Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The Imd signaling cascade, similar to the mammalian TNF-receptor pathway, controls antimicrobial peptide expression in Drosophila. We performed a large-scale RNAi screen to identify novel components of the Imd pathway in Drosophila S2 cells. In all, 6713 dsRNAs from an S2 cell-derived cDNA library were analyzed for their effect on Attacin promoter activity in response to Escherichia coli. We identified seven gene products required for the Attacin response in vitro, including two novel Imd pathway components: inhibitor of apoptosis 2 (Iap2) and transforming growth factor-activated kinase 1 (TAK1)-binding protein (TAB). Iap2 is required for antimicrobial peptide response also by the fat body in vivo. Both these factors function downstream of Imd. Neither TAB nor Iap2 is required for Relish cleavage, but may be involved in Relish nuclear localization in vitro, suggesting a novel mode of regulation of the Imd pathway. Our results show that an RNAi-based approach is suitable to identify genes in conserved signaling cascades.

Ort, förlag, år, upplaga, sidor
2005. Vol. 24, nr 19, s. 3423-34
Nyckelord [en]
Adaptor Proteins; Signal Transducing/*genetics/metabolism, Amino Acid Sequence, Animals, Base Sequence, Blotting; Western, Cells; Cultured, DNA/genetics, DNA Primers, Drosophila Proteins/*genetics/immunology/*metabolism, Drosophila melanogaster/*genetics/immunology/microbiology, Electrophoresis; Polyacrylamide Gel, Escherichia coli/immunology, Gene Library, Immunohistochemistry, Inhibitor of Apoptosis Proteins/*genetics/metabolism, Insect Proteins/metabolism, Luciferases, Models; Biological, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, RNA Interference, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis; DNA, Signal Transduction/*genetics/immunology
Identifikatorer
URN: urn:nbn:se:umu:diva-17051PubMedID: 16163390OAI: oai:DiVA.org:umu-17051DiVA, id: diva2:156724
Tillgänglig från: 2007-10-28 Skapad: 2007-10-28 Senast uppdaterad: 2018-06-09Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

PubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=16163390&dopt=Citation

Personposter BETA

Stöven, SvenjaHultmark, Dan

Sök vidare i DiVA

Av författaren/redaktören
Stöven, SvenjaHultmark, Dan
Av organisationen
Umeå centrum för molekylär patogenes (UCMP) (Medicinska fakulteten)Klinisk mikrobiologiKlinisk bakteriologi

Sök vidare utanför DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetricpoäng

pubmed
urn-nbn
Totalt: 333 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf