Functional diversity of the Drosophila PGRP-LC gene cluster in the response to lipopolysaccharide and peptidoglycan.
2003 (English)In: J Biol Chem, ISSN 0021-9258, Vol. 278, no 29, 26319-22 p.Article in journal (Refereed) Published
The peptidoglycan recognition protein PGRP-LC is a major activator of the imd/Relish pathway in the Drosophila immune response. Three transcripts are generated by alternative splicing of the complex PGRP-LC gene. The encoded transmembrane proteins share an identical intracellular part, but each has a separate extracellular PGRP-domain: x, y, or a. Here we show that two of these isoforms play unique roles in the response to different microorganisms. Using RNA interference in Drosophila mbn-2 cells, we found that PGRP-LCx is the only isoform required to mediate signals from Gram-positive bacteria and purified bacterial peptidoglycan. By contrast, the recognition of Gram-negative bacteria and bacterial lipopolysaccharide requires both PGRP-LCa and LCx. The third isoform, LCy, is expressed at lower levels and may be partially redundant. Two additional PGRP domains in the gene cluster, z and w, are both included in a single transcript of a separate gene, PGRP-LF. Suppression of this transcript does not block the response to any of the microorganisms tested.
Place, publisher, year, edition, pages
2003. Vol. 278, no 29, 26319-22 p.
Alternative Splicing, Animals, Carrier Proteins/*genetics, Cell Line, Drosophila/drug effects/*genetics/immunology/microbiology, Drosophila Proteins/*genetics, Genes; Insect/drug effects, Gram-Negative Bacteria/pathogenicity, Gram-Positive Bacteria/pathogenicity, Lipopolysaccharides/pharmacology, Multigene Family/drug effects, Peptidoglycan/pharmacology, RNA Interference
IdentifiersURN: urn:nbn:se:umu:diva-17058DOI: 10.1074/jbc.C300184200PubMedID: 12777387OAI: oai:DiVA.org:umu-17058DiVA: diva2:156731