Cloning, mapping and mutational analysis of the S-adenosylmethionine decarboxylase gene in Drosophila melanogaster.
1997 (English)In: Mol Gen Genet, ISSN 0026-8925, Vol. 256, no 6, 652-60 p.Article in journal (Other academic) Published
S-adenosylmethionine decarboxylase is a key enzyme in the synthesis of polyamines. These small cationic molecules are required for growth and development in all organisms. A wealth of biological processes, including synthesis of DNA and protein and condensation of chromatin, involve polyamines. Inhibition of polyamine synthesis has been proposed for treatment of cancer but this requires more knowledge about the in vivo function of polyamines. We report here the cloning of the S-adenosylmethionine decarboxylase gene from Drosophila melanogaster and the analysis of corresponding mutants. The mutant phenotypes are similar to those previously described for ribosomal protein genes (Minutes) and rRNA genes (bobbed). This work elucidates the in vivo consequences of impaired polyamine synthesis with respect to the development of a whole animal.
Place, publisher, year, edition, pages
1997. Vol. 256, no 6, 652-60 p.
Adenosylmethionine Decarboxylase/*genetics, Amino Acid Sequence, Animal Population Groups/genetics, Animals, Base Sequence, Chromatin/genetics/ultrastructure, Chromosome Mapping, Cloning; Molecular, DNA Mutational Analysis, Drosophila melanogaster/enzymology/*genetics, Genes; Insect, Genetic Complementation Test, Insect Proteins/*genetics, Molecular Sequence Data, Phenotype, Plants/genetics, Polyamines/metabolism, Sequence Alignment, Sequence Homology; Amino Acid, Species Specificity
IdentifiersURN: urn:nbn:se:umu:diva-17254PubMedID: 9435790OAI: oai:DiVA.org:umu-17254DiVA: diva2:156927