Genetic organization of the ci-M-pan region on chromosome IV in Drosophila melanogaster.
1999 (English)In: Genome, ISSN 0831-2796, E-ISSN 1480-3321, Vol. 42, no 6, 1144-1149 p.Article in journal (Refereed) Published
The genes cubitus interruptus (ci), ribosomal protein S3A (RpS3A), and pangolin (pan) are localized within 73 kb in the cytological region 101F-102A on chromosome IV in Drosophila melanogaster. A region of 13 kb harbours the regulatory regions of both ci and pan, transcribed in opposite directions, and a 1.1-kb gene encoding RpS3A. This dense clustering gives rise to very complicated complementation patterns between different alleles in these loci. We investigated this region genetically and molecularly by use of an enhancer trap line (IA5), where the P-element was found to be inserted into the first intron of pan. Screens for imprecise excisions of the P-element were performed, and complementations between new and old established mutant lines were investigated. We found that when mutated or deleted the RpS3A gene gives rise to a Minute phenotype, and we conclude that M(4)101 encodes the ribosomal protein S3A.
Place, publisher, year, edition, pages
1999. Vol. 42, no 6, 1144-1149 p.
Animals, Blotting; Northern, DNA Transposable Elements, DNA-Binding Proteins/*genetics, Drosophila Proteins, Drosophila melanogaster/embryology/*genetics, Female, Genes; Insect, Genetic Complementation Test, Insect Proteins/*genetics, Male, Repressor Proteins, Ribosomal Proteins/*genetics, Transcription Factors/*genetics, beta-Galactosidase/genetics
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-17911PubMedID: 10659782OAI: oai:DiVA.org:umu-17911DiVA: diva2:157584