Chloride binding by the AML1/Runx1 transcription factor studied by NMR
2001 (English)In: FEBS Letters, ISSN 0014-5793, Vol. 488, no 1-2, 81-4 p.Article in journal (Refereed) Published
It is known that the DNA binding Runt domain of the AML1/Runx1 transcription factor coordinates Cl(-) ions. In this paper we have determined Cl(-) binding affinities of AML1 by (35)Cl nuclear magnetic resonance (NMR) linewidth analysis. The Runt domain binds Cl(-) with a dissociation constant (K(d,Cl)) of 34 mM. If CBFbeta is added to form a 1:1 complex, the K(d,Cl) value increases to 56 mM. Homology modeling suggests that a high occupancy Cl(-) binding site overlaps with the DNA binding surface. NMR data show that DNA displaces this Cl(-) ion. Possible biological roles of Cl(-) binding are discussed based on these findings.
Place, publisher, year, edition, pages
2001. Vol. 488, no 1-2, 81-4 p.
AML1, Runt domain, 35Cl, Nuclear magnetic resonance spectroscopy, Anion binding to protein
IdentifiersURN: urn:nbn:se:umu:diva-17923DOI: doi:10.1016/S0014-5793(00)02390-5PubMedID: 11163800OAI: oai:DiVA.org:umu-17923DiVA: diva2:157596