Change search
ReferencesLink to record
Permanent link

Direct link
Disruption of quorum sensing in seawater abolishes attraction of zoospores of the green alga Ulva to bacterial biofilms.
Umeå University, Faculty of Science and Technology, Molecular Biology (Faculty of Science and Technology).
Show others and affiliations
2005 (English)In: Environ Microbiol, ISSN 1462-2912, Vol. 7, no 2, 229-40 p.Article in journal (Refereed) Published
Abstract [en]

Zoospores of the eukaryotic green seaweed Ulva respond to bacterial N-acylhomoserine lactone (AHL) quorum sensing signal molecules for the selection of surface sites for permanent attachment. In this study we have investigated the production and destruction of AHLs in biofilms of the AHL-producing marine bacterium, Vibrio anguillarum and their stability in seawater. While wild type V. anguillarum NB10 was a strong attractor of zoospores, inactivation of AHL production in this strain by either expressing the recombinant Bacillus lactonase coding gene aiiA, or by mutating the AHL biosynthetic genes, resulted in the abolition of zoospore attraction. In seawater, with a pH of 8.2, the degradation of AHL molecules was temperature-dependent, indicating that the AHLs produced by marine bacterial biofilms have short half-lives. The Ulva zoospores sensed a range of different AHL molecules and in particular more zoospores settled on surfaces releasing AHLs with longer (>six carbons) N-linked acyl chains. However, this finding is likely to be influenced by the differential diffusion rates of AHLs from the experimental surface matrix. Molecules with longer N-acyl chains, such as N-(3-oxodecanoyl)- L-homoserine lactone, diffused more slowly than those with shorter N-acyl chains such as N-(3-hydroxy-hexanoyl)- L-homoserine lactone. Image analysis using GFP-tagged V. anguillarum biofilms revealed that spores settle directly on bacterial cells and in particular on microcolonies which we show are sites of concentrated AHL production.

Place, publisher, year, edition, pages
2005. Vol. 7, no 2, 229-40 p.
Keyword [en]
4-Butyrolactone/*analogs & derivatives/analysis/*biosynthesis/chemistry/pharmacology, Bacterial Proteins/genetics/metabolism, Biofilms, Cell Adhesion/*physiology, Chemotactic Factors/*pharmacology, Diffusion, Homoserine/*analogs & derivatives/chemistry, Hydrogen-Ion Concentration, Metalloendopeptidases/genetics/metabolism, Mutation, Seawater/chemistry, Temperature, Ulva/*physiology, Vibrio/*metabolism
URN: urn:nbn:se:umu:diva-17993PubMedID: 15658990OAI: diva2:157666
Available from: 2007-11-26 Created: 2007-11-26 Last updated: 2011-01-12Bibliographically approved

Open Access in DiVA

No full text

Other links


Search in DiVA

By author/editor
Milton, Debra L
By organisation
Molecular Biology (Faculty of Science and Technology)

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 38 hits
ReferencesLink to record
Permanent link

Direct link