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Analysis of protein and lipid interactions using liposome co-sedimentation assays
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
2020 (English)In: Caveolae: methods and protocols / [ed] Cedric M. Blouin, Humana Press, 2020, , p. 9p. 119-127Chapter in book (Refereed)
Abstract [en]

The dynamic assembly of proteins at the membrane interphase is key to many cell biological processes such as the generation and stabilization of caveolae at the cell surface via coat proteins. The liposome co-sedimentation assay has been widely used for studies of protein and lipid interactions and has provided important information about binding mechanisms, lipid-binding specificity, and curvature preference of proteins. Here, we describe this technique in detail and how it can be used as a tool to address the membrane-binding ability and lipid specificity of caveolae-associated proteins.

Place, publisher, year, edition, pages
Humana Press, 2020. , p. 9p. 119-127
Series
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 2169
Keywords [en]
Caveolae coat, Cavin, Co-sedimentation, EHD2, Lipid specificity, Liposome pull down, Liposomes, Protein and lipid interactions, SUVs
National Category
Biophysics Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-197951DOI: 10.1007/978-1-0716-0732-9_11Scopus ID: 2-s2.0-85086686139ISBN: 978-1-0716-0731-2 (print)ISBN: 978-1-0716-0734-3 (print)ISBN: 978-1-0716-0732-9 (electronic)OAI: oai:DiVA.org:umu-197951DiVA, id: diva2:1682173
Available from: 2022-07-08 Created: 2022-07-08 Last updated: 2023-03-24Bibliographically approved

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Larsson, ElinHubert, MadlenLundmark, Richard

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Total: 129 hits
CiteExportLink to record
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Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
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Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf