Regulation of the Drosophila engrailed gene by Polycomb repressor complex 2
2009 (English)In: Mechanisms of Development, ISSN 0925-4773, E-ISSN 1872-6356, Vol. 126, no 5-6, 443-448 p.Article in journal (Refereed) Published
Suppressor-of-zeste-12 (Su(z)12) is a core component of the Polycomb repressive complex 2 (PRC2), which has a methyltransferase activity directed towards lysine residues of histone 3. Mutations in Polycomb group (PcG) genes cause de-repression of homeotic genes and subsequent homeotic transformations. Another target for Polycomb silencing is the engrailed gene, which encodes a key regulator of segmentation in the early Drosophila embryo. In close proximity to the en gene is a Polycomb Response Element, but whether en is regulated by Su(z)12 is not known. In this report, we show that en is not de-repressed in Su(z)12 or Enhancer-of-zeste mutant clones in the anterior compartment of wing discs. Instead, we find that en expression is down-regulated in the posterior portion of wing discs, indicating that the PRC2 complex acts as an activator of en. Our results indicate that this is due to secondary effects, probably caused by ectopic expression of Ubx and Abd-B.
Place, publisher, year, edition, pages
Amsterdam: Elsevier, 2009. Vol. 126, no 5-6, 443-448 p.
Gene regulation, Su(z)12, Engrailed, Wing disc, Drosophila
Cell and Molecular Biology Developmental Biology
IdentifiersURN: urn:nbn:se:umu:diva-18646DOI: 10.1016/j.mod.2009.01.004ISI: 000266975100014OAI: oai:DiVA.org:umu-18646DiVA: diva2:174303