Change search
ReferencesLink to record
Permanent link

Direct link
Actin is bundled in activation-tagged tobacco mutants that tolerate aluminum
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
2007 (English)In: Planta, ISSN 0032-0935, E-ISSN 1432-2048, Vol. 225, no 2, 451-468 p.Article in journal (Refereed) Published
Abstract [en]

A panel of aluminum-tolerant (AlRes) mutants was isolated by protoplast-based T-DNA activation tagging in the tobacco cultivar SR1. The mutants fell into two phenotypic classes: a minority of the mutants were fertile and developed similarly to the wild type (type I), the majority was male-sterile and grew as semi-dwarfs (type II). These traits, along with the aluminum tolerance, were inherited in a monogenic dominant manner. Both types of mutants were characterized by excessive bundling of actin microfilaments and by a strongly increased abundance of actin, a phenotype that could be partially phenocopied in the wild type by treatment with aluminum chloride. The actin bundles could be dissociated into finer strands by addition of exogenous auxin in both types of mutants. However, actin microfilaments and leaf expansion were sensitive to blockers of actin assembly in the wild type and in the mutants of type I, whereas they were more tolerant in the mutants of type II. The mutants of type II displayed a hypertrophic development of vasculature, manifest in form of supernumerary leaf veins and extended xylem layers in stems and petioles. Whereas mutants of type I were characterized by a normal, but aluminum-tolerant polar auxin-transport, auxin-transport was strongly promoted in the mutants of type II. The phenotype of these mutants is discussed in terms of reduced endocytosis leading, concomitantly with aluminum tolerance, to changes in polar auxin transport.

Place, publisher, year, edition, pages
New York: Springer, 2007. Vol. 225, no 2, 451-468 p.
Keyword [en]
Actin microfilaments, Activation tagging, Aluminum tolerance, Auxin transport male sterility tobacco (Nicotiana tabacum L.)
National Category
Plant Biotechnology
URN: urn:nbn:se:umu:diva-18891DOI: 10.1007/s00425-006-0359-0ISI: 000242855000017PubMedID: 16909289OAI: diva2:175066
Available from: 2009-02-26 Created: 2009-02-26 Last updated: 2016-08-15Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Ahad, Abdul
By organisation
Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
In the same journal
Plant Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 67 hits
ReferencesLink to record
Permanent link

Direct link