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HPLC-ESI-MS/MS of imidazole alkaloids in Pilocarpus microphyllus
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Umeå University, Faculty of Science and Technology, Department of Plant Physiology.
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2008 (English)In: Molecules, ISSN 1420-3049, Vol. 13, no 7, 1518-29 p.Article in journal (Refereed) Published
Abstract [en]

Pilocarpine, an important imidazole alkaloid, is extracted from the leaves of Pilocarpus microphyllus (Rutaceae), known in Brazil as jaborandi and used mainly for the treatment of glaucoma. Jaborandi leaves also contain other imidazole alkaloids, whose pharmacological and physiological properties are unknown, and whose biosynthetic pathways are under investigation. In the present study, a HPLC method coupled with ESI-MS(n) was developed for their qualitative and quantitative analysis. This method permits the chromatographic separation of the imidazole alkaloids found in extracts of jaborandi, as well as the MS/MS analysis of the individual compounds. Thus two samples: leaves of P. microphyllus and a paste that is left over after the industrial extraction of pilocarpine; were compared. The paste was found to contain significant amounts of pilocarpine and other imidazole alkaloids, but had a slightly different alkaloid profile than the leaf extract. The method is suitable for the routine analysis of samples containing these alkaloids, as well as for the separation and identification of known and novel alkaloids from this family, and may be applied to further studies of the biosynthetic pathway of pilocarpine in P. microphyllus.

Place, publisher, year, edition, pages
Basel, Switzerland: MDPI AG , 2008. Vol. 13, no 7, 1518-29 p.
Keyword [en]
Jaborandi, Pilocarpus microphyllus, imidazole alkaloids, pilocarpine, HPLCESI- MS/MS
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URN: urn:nbn:se:umu:diva-18896PubMedID: 18719522OAI: diva2:175073
Available from: 2009-02-26 Created: 2009-02-26 Last updated: 2013-02-04Bibliographically approved

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Abreu, Ilka NacifAndreazza, Nathalia LuizaMazzafera, Paulo
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Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
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