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Production of imidazole alkaloids in cell cultures of jaborandi as affected by the medium pH
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
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2009 (English)In: Biotechnology letters, ISSN 0141-5492, E-ISSN 1573-6776, Vol. 31, no 4, 607-614 p.Article in journal (Refereed) Published
Abstract [en]

The effect of pH (from 4.8 to 9.8) on the production of pilosine and pilocarpine and on their partition between cell and medium was studied in two lineages (P and PP) of Pilocarpus microphyllus cell suspension cultures. Highest mass accumulation was observed at high pHs and both lineages produced pilocarpine while only lineage PP produced pilosine. Both alkaloids were released in the medium but higher accumulation occurred in the cells. The highest production of pilocarpine was at pH 8.8-9.8 in both cell lineages. Other imidazole alkaloids were also identified in both lineages. At all pHs tested, the pH in the media cultures tended to stabilize around 6 after 10-15 days of cultivation. NO(3) (-) and NH(4) (+) variation in the media might partially explain the pH stabilization.

Place, publisher, year, edition, pages
Springer, 2009. Vol. 31, no 4, 607-614 p.
National Category
Agricultural Biotechnology
URN: urn:nbn:se:umu:diva-18899DOI: 10.1007/s10529-008-9895-yPubMedID: 19066732OAI: diva2:175077
Available from: 2009-02-26 Created: 2009-02-26 Last updated: 2013-02-04Bibliographically approved

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Abreu, Ilka Nacif
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Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
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