We study symmetries in the phase plane for separable, autonomous two-state systems of ordinary differential equations (ODEs). We prove two main theoretical results concerning the existence and non-triviality of two orthogonal symmetries for such systems. In particular, we show that these symmetries correspond to translations in the internal energy of the system, and describe their action on solution trajectories in the phase plane. In addition, we apply recent results establishing how phase plane symmetries can be extended to incorporate temporal dynamics to these energy translation symmetries. Subsequently, we apply our theoretical results to the analysis of three models from the field of mathematical biology: a canonical biological oscillator model, the Lotka–Volterra (LV) model describing predator–prey dynamics, and the SIR model describing the spread of a disease in a population. We describe the energy translation symmetries in detail, including their action on biological observables of the models, derive analytic expressions for the extensions to the time domain, and discuss their action on solution trajectories.