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In this study, the magnitude of stomatal water loss was compared between different Arabidopsis thaliana mutants. The stomatal water loss among different mutants of Populus sp. was also examined in this study. Fourteen different Arabidopsis thaliana mutants were sown and later genotyped using DNA-extraction, PCR, and gel electrophoresis. Leaf water loss analysis was made on both Arabidopsis thaliana and Populus sp. The data was then used in statistical tests, to find if there was a significant difference in rate of stomatal water loss between different genotypes. The result showed a significant difference in water loss when comparing the toc1-5 and T89 genotypes and the toc1-5 and 1119-4 genotypes in Populus sp. There was no significant difference between the 1119-4 and T89 genotypes in Populus sp. The result also showed that there was a significant difference between 25 combinations of the Arabidopsis thaliana genotypes. A conclusion was that TOC1 reduces water loss in Arabidopsis thaliana, indicating a role in stomatal clousure. Future research is needed to examine differences between genotypes among both species included in the study.