Gas chromatography-mass spectrometry determination of the pentafluorobenzoyl derivative of methylhydrazine in false morel (Gyromitra esculenta) as a monitor for the content of the toxin gyromitrin
2006 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1125, no 2, 229-233 p.Article in journal (Refereed) Published
The main toxic compound found in false morel (Gyromitra esculenta) is acetaldehyde-N-methyl-N-formylhydrazone (gyromitrin). This paper describes a method of determining the total hydrazones content based on acid hydrolysis of gyromitrin and other related hydrazones in air-dried false morel followed by derivatisation of methylhydrazine with pentafluorobenzoyl chloride. The derivative, tris-pentafluorobenzoyl methylhydrazine (tris-PFB-MH) is analyzed by gas chromatography–mass spectrometry. The overall precision of the method is better than 10% (relative standard deviation) for 0.5 ng/μl methylhydrazine in solution. The minimum detectable concentration of methylhydrazine (tris-PFB-MH) by this method is estimated to be approximately 12 pg/μl, which is equal to 0.3 μg/g dry matter (DM) of false morel.
Place, publisher, year, edition, pages
Amsterdam: Elsevier, 2006. Vol. 1125, no 2, 229-233 p.
False morel, Gyromitra esculenta, GC–MS, Tris-pentafluorobenzoyl methylhydrazine, Methylhydrazine, Gyromitrin
Biochemistry and Molecular Biology Analytical Chemistry
IdentifiersURN: urn:nbn:se:umu:diva-18983DOI: 10.1016/j.chroma.2006.05.040ISI: 000239974200012OAI: oai:DiVA.org:umu-18983DiVA: diva2:201079