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Fatty acid profile of the erythrocyte membrane preceding development of Type 2 diabetes mellitus.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Occupational and Environmental Medicine.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Epidemiology and Global Health.ORCID iD: 0000-0003-2475-7131
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Nutritional Research.
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2008 (English)In: Nutrition, metabolism, and cardiovascular diseases : NMCD, ISSN 1590-3729, Vol. 18, no 7, 503-510 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND AND AIMS: The respective roles of dietary fatty acids in the pathogenesis of diabetes are as yet unclear. Erythrocyte membrane fatty acid (EMFA) composition may provide an estimate of dietary fatty acid intake. This study investigates the relation between EMFA composition and development of Type 2 diabetes mellitus. METHODS AND RESULTS: In a nested case-referent design we studied 159 individuals tested as non-diabetic at baseline who after a mean observation time of 5.4+/-2.6years were diagnosed with Type 2 diabetes mellitus and 291 sex- and age-matched referents. Higher proportions of pentadecanoic acid (15:0) and heptadecanoic acid (17:0) were associated with a lower risk of diabetes. In accordance with earlier findings, higher proportions of palmitoleic (16:1 n-7), dihomo-gamma-linolenic (20:3 n-6) and adrenic (22:4 n-6) acids were associated with increased risk, whereas linoleic (18:2 n-6) and clupanodonic (22:5 n-3) acids were inversely associated with diabetes. After adjustment for BMI, HbA1c, alcohol intake, smoking and physical activity the only significant predictors were 15:0 and 17:0 as protective factors and 22:4 n6 as risk factor. CONCLUSION: In accordance with previous studies, our results indicate that EMFA-patterns predict development of Type 2 diabetes mellitus. The inverse association with two saturated fatty acids, previously shown to reflect consumption of dairy products, is a new finding.

Place, publisher, year, edition, pages
2008. Vol. 18, no 7, 503-510 p.
National Category
Environmental Health and Occupational Health
URN: urn:nbn:se:umu:diva-19668DOI: 10.1016/j.numecd.2007.04.005PubMedID: 18042359OAI: diva2:202273
Available from: 2009-03-09 Created: 2009-03-09 Last updated: 2015-04-29
In thesis
1. Diet and Cardiometabolic Disease: Dietary trends and the impact of diet on diabetes and cardiovascular disease
Open this publication in new window or tab >>Diet and Cardiometabolic Disease: Dietary trends and the impact of diet on diabetes and cardiovascular disease
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Cardiovascular diseases are the leading cause of death in most industrialised countries and in developing countries the trend in cardiovascular-related deaths is increasing. World-wide, type 2 diabetes mellitus (T2DM) is an emerging cause of disability and premature death. Both these conditions are closely associated with the consumption of energy-dense foods and food products that are poor in nutrients, as well as with a sedentary lifestyle. Pharmacological and surgical interventions can improve the outcome and delay the progression of the disease, but in terms of population-level prevention there is no substitute for the adoption of a healthy lifestyle.


The underlying studies were conducted in Västerbotten (the VIP study), and in Norrbotten and Västerbotten combined (the MONICA Project). Norrbotten andVästerbotten are the two northernmost counties in Sweden. Since the mid-1980sthe prevalence of cardiovascular disease has decreased and diabetes rates haveremained stable in this region, despite of an unbroken trend of increasing body weight.


The aim of this thesis is to describe changes in reported dietary habits, estimatetheir relative importance as risk factors for diabetes and cardiovascular disease, and finally to identify lifestyle components as potential targets for intervention.


The first paper describes changes in self-reported food consumption between 1986 and 1999. During this period, the population in question switched from products with high saturated fatty acid content (e.g. milk containing 3% fat, butter) to foods containing less saturated fat (e.g. milk containing 1.5% fat, vegetable oil, low-fat margarine); pasta and rice were consumed more often, and potatoes were consumed less. Convenience foods (e.g. hamburgers, snacks, sweets) became more popular, whilst traditional dishes (e.g. potato dumplings, black pudding, blöta) decreased in popularity. Fruit and vegetable intake remained low. In paper two we study the effects of these changes in food intake on the risk of developing T2DM using body fat distribution as an early indicator. Increased consumption of convenience foods was associated with unfavourable changes (smaller hip circumference and larger waist circumference), whereas the increased consumption of vegetable oil and pasta was associated with low-risk fat distribution. In the third paper we report studies on the association between fat consumption and T2DM. We used the pattern of fatty acids in the membranes of red blood cells as a marker of fat intake. In addition to confirming earlier findings (markers of the intake of saturated fat are associated with increased risk of T2DM and markers of unsaturated fat are associated with reduced T2DM risk), we also identified associations between two markers of milk-derived saturated fat intake and enterolactone, a biomarker of dietary fibre intake, and the risk of developing myocardial infarction. Our results indicate that moderately high levels of enterolactone intake in men are associated with lower risk of experiencing myocardial infarction. Manuscript 5 ranks education level, physical activity, smoking status, and self-reported intake of dietary fibre and fatty acids according to their effects on body fat distribution. Increased levels of physical activity, a higher education level and a reduced intake of saturated fat from meat were ranked as the most strongly associated factors in both men and women. Increased intake of dietary fibre from grains in women, and increased intake of dietary fibre from fruits and vegetables in men, was also inversely associated with average waist circumference.


Both questionnaire-based and biological markers of the risk of developing diabetes or cardiovascular disease have been identified. Based on available population level measurements, reduced consumption of convenience foods, increased consumption of whole-grain products, fruits and vegetables, vegetable oil and pasta as well as increased physical activity are potential goals for interventions in northern Sweden.

Place, publisher, year, edition, pages
Umeå: Folkhälsa och klinisk medicin, 2007. 81 p.
Umeå University medical dissertations, ISSN 0346-6612 ; 1108
Medicine, Cross-sectional study, Cross-sectional survey, Diet, Dietary intake, Dietary survey, Food consumption, Food frequency, Milk, MONICA, Sweden, Body Mass Index, Hip circumference, Waist circumference, Diabetes, Metabolic syndrome, Cardiometabolic syndrome, Cardiovascular disease, Erythrocyte Membrane, Fatty Acids, Membrane Lipids, Fatty acid desaturases, Pentadecanoic acid, Heptadecanoic acid, Lignan, Enterolactone, Dietary Fibre, Physical activity, Education, Smoking, Alcohol, Medicin
Research subject
urn:nbn:se:umu:diva-1369 (URN)978-91-7264-354-3 (ISBN)
Public defence
2007-10-12, sal B, 1 D, Tandläkarhögskolan, Norrlands Universitetssjukhus, Umeå, 09:00 (English)
Available from: 2007-09-19 Created: 2007-09-19 Last updated: 2009-05-27Bibliographically approved

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Krachler, BennoNorberg, MargaretaJohansson, IngegerdWeinehall, LarsLindahl, Bernt
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