Change search
ReferencesLink to record
Permanent link

Direct link
Maximal transcription of aur (aureolysin) and sspA (serine protease) in Staphylococcus aureus requires staphylococcal accessory regulator R (sarR) activity.
Umeå University, Faculty of Medicine, Odontology. Umeå University, Faculty of Medicine, Odontology, Oral Microbiology.
2008 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, Vol. 284, no 2, 158-64 p.Article in journal (Refereed) Published
Abstract [en]

Previous studies have shown that expression of aur (metalloprotease; aureolysin) and sspA (V8 protease; serine protease) in Staphylococcus aureus strain 8325-4 is maximal in the postexponential phase of growth, when the agr (RNAIII) system is activated. Transcription of aur and sspA is mainly regulated through repression by sarA and rot, and RNAIII stimulates protease production by inhibiting translation of rot mRNA. As SarR is a repressor of sarA, inactivation of sarR would result in downregulation of aur and sspA transcription. This was confirmed by mRNA analysis using quantitative real-time PCR. However, we found that sarR acted as a direct stimulator, i.e. its positive effect on aur and sspA transcription did not require sarA (or rot) per se. In addition, aur and sspA were dependent on sarR for maximal transcription. This stimulating role of sarR was not restricted to the rsbU-deficient laboratory strain 8325-4 but was also demonstrated in S. aureus strain SH1000 (rsbU-complemented derivative of 8325-4) and in one clinical isolate.

Place, publisher, year, edition, pages
2008. Vol. 284, no 2, 158-64 p.
URN: urn:nbn:se:umu:diva-20470DOI: 10.1111/j.1574-6968.2008.01198.xPubMedID: 18576947OAI: diva2:208781
Available from: 2009-03-20 Created: 2009-03-20 Last updated: 2009-03-20

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Oscarsson, Jan
By organisation
OdontologyOral Microbiology
In the same journal
FEMS Microbiology Letters

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 45 hits
ReferencesLink to record
Permanent link

Direct link