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Kluyveromyces lactis γ-toxin, a ribonuclease that recognizes the anticodon stem loop of tRNA
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology). (Byström)
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology). (Byström)
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology). (Byström)
2008 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 36, no 4, 1072-1080 p.Article in journal (Refereed) Published
Abstract [en]

Kluyveromyces lactis gamma-toxin is a tRNA endonuclease that cleaves Saccharomyces cerevisiae [see text] between position 34 and position 35. All three substrate tRNAs carry a 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U) residue at position 34 (wobble position) of which the mcm(5) group is required for efficient cleavage. However, the different cleavage efficiencies of mcm(5)s(2)U(34)-containing tRNAs suggest that additional features of these tRNAs affect cleavage. In the present study, we show that a stable anticodon stem and the anticodon loop are the minimal requirements for cleavage by gamma-toxin. A synthetic minihelix RNA corresponding to the anticodon stem loop (ASL) of the natural substrate [see text] is cleaved at the same position as the natural substrate. In [see text], the nucleotides U(34)U(35)C(36)A(37)C(38) are required for optimal gamma-toxin cleavage, whereas a purine at position 32 or a G in position 33 dramatically reduces the cleavage of the ASL. Comparing modified and partially modified forms of E. coli and yeast [see text] reinforced the strong stimulatory effects of the mcm(5) group, revealed a weak positive effect of the s(2) group and a negative effect of the bacterial 5-methylaminomethyl (mnm(5)) group. The data underscore the high specificity of this yeast tRNA toxin.

Place, publisher, year, edition, pages
2008. Vol. 36, no 4, 1072-1080 p.
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Medical and Health Sciences
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URN: urn:nbn:se:umu:diva-20534DOI: 10.1093/nar/gkm1121PubMedID: 18096622OAI: oai:DiVA.org:umu-20534DiVA: diva2:208858
Available from: 2009-03-20 Created: 2009-03-20 Last updated: 2012-12-03

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Esberg, AndersByström, Anders S

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