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Global regulation of the interphase microtubule system by abundantly expressed Op18/stathmin.
Umeå University, Faculty of Medicine, Molecular Biology (Faculty of Medicine). (Gullberg)
Umeå University, Faculty of Medicine, Molecular Biology (Faculty of Medicine). (Gullberg)
Umeå University, Faculty of Medicine, Molecular Biology (Faculty of Medicine). (Gullberg)
Umeå University, Faculty of Medicine, Molecular Biology (Faculty of Medicine). (Gullberg)
2008 (English)In: Molecular biology of the cell, ISSN 1939-4586, Vol. 19, no 7, 2897-906 p.Article in journal (Refereed) Published
Abstract [en]

Op18/stathmin (Op18), a conserved microtubule-depolymerizing and tubulin heterodimer-binding protein, is a major interphase regulator of tubulin monomer-polymer partitioning in diverse cell types in which Op18 is abundant. Here, we addressed the question of whether the microtubule regulatory function of Op18 includes regulation of tubulin heterodimer synthesis. We used two human cell model systems, K562 and Jurkat, combined with strategies for regulatable overexpression or depletion of Op18. Although Op18 depletion caused extensive overpolymerization and increased microtubule content in both cell types, we did not detect any alteration in polymer stability. Interestingly, however, we found that Op18 mediates positive regulation of tubulin heterodimer content in Jurkat cells, which was not observed in K562 cells. By analysis of cells treated with microtubule-poisoning drugs, we found that Jurkat cells regulate tubulin mRNA levels by a posttranscriptional mechanism similarly to normal primary cells, whereas this mechanism is nonfunctional in K562 cells. We present evidence that Op18 mediates posttranscriptional regulation of tubulin mRNA in Jurkat cells through the same basic autoregulatory mechanism as microtubule-poisoning drugs. This, combined with potent regulation of tubulin monomer-polymer partitioning, enables Op18 to exert global regulation of the microtubule system.

Place, publisher, year, edition, pages
2008. Vol. 19, no 7, 2897-906 p.
Identifiers
URN: urn:nbn:se:umu:diva-20781DOI: 10.1091/mbc.E08-01-0058PubMedID: 18434595OAI: oai:DiVA.org:umu-20781DiVA: diva2:209535
Available from: 2009-03-25 Created: 2009-03-25 Last updated: 2011-10-21
In thesis
1. Cytoskeletal filament systems: assembly, regulation, and interplay in mammalian cells
Open this publication in new window or tab >>Cytoskeletal filament systems: assembly, regulation, and interplay in mammalian cells
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The cell represents the basic unit of structure and function for all life. The interior of a eukaryotic cell is organized by an extensive array of protein filaments – collectively referred to as the cytoskeleton. These filaments serve diverse essential functions, e.g. to provide mechanical resilience, facilitate intracellular transport, and enable cell polarization, locomotion and division. Here I have explored the mechanisms that regulate synthesis and assembly of two cytoskeletal filament systems – microtubules and septins – and how these interact in human cells. The present thesis is based on three principal discoveries. Firstly, we have found that the microtubule-destabilizing protein Op18/Stathmin also regulates synthesis of tubulin heterodimers, which are the building blocks for microtubules. Secondly, we have unraveled the general rules that govern assembly of mammalian septins into native polymerization-competent heterooligomers. Finally, our combined results point to a non-reciprocal interplay whereby interphase microtubules support a disc-like arrangement of septin filaments, which delineate static plasma membrane regions. I here discuss the physiological significance and implications of these findings.

Place, publisher, year, edition, pages
Umeå: Umeå universitet, Institutionen för molekylärbiologi, 2011. 68 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 1450
National Category
Cell and Molecular Biology
Research subject
cellforskning
Identifiers
urn:nbn:se:umu:diva-48508 (URN)978-91-7459-301-3 (ISBN)
Public defence
2011-11-11, Major Groove, Institutionen för Molekylärbiologi, Försörjningsvägen, Byggnad 6L, Umeå Universitet, Umeå, 09:00 (English)
Opponent
Supervisors
Available from: 2011-10-21 Created: 2011-10-20 Last updated: 2011-10-21Bibliographically approved

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