A comparative study on ganglioside micelles using electronic energy transfer, fluorescence correlation spectroscopy and light scattering techniques
2009 (English)In: Physical Chemistry, Chemical Physics - PCCP, ISSN 1463-9076, E-ISSN 1463-9084, Vol. 11, no 21, 4335-4343 p.Article in journal (Refereed) Published
Ganglioside (GM1) micelles have been studied by means of three different techniques: fluorescence correlation spectroscopy (FCS), electronic energy transfer, as monitored by time-resolved fluorescence spectroscopy, as well as static and dynamic light scattering. The aggregation numbers obtained, 168 ± 4, remain constant over a wide range of GM1 concentrations (0.764-156 M), are very consistent when using different donor-acceptor energy transfer pairs and have served as reference values in tests of the FCS method. It is recommended to calibrate the focal volume by using known dye concentrations. For this the rhodamine dye, 5-TAMRA, turns out to be most suitable. It is also shown that FCS provides correct values of the aggregation numbers, provided that the focal volume is calibrated by using updated values of the diffusion constant of Rhodamine 6G. These results also support recent methodological advances in FCS.
Place, publisher, year, edition, pages
Royal Society of Chemistry , 2009. Vol. 11, no 21, 4335-4343 p.
IdentifiersURN: urn:nbn:se:umu:diva-20816DOI: 10.1039/b821658dOAI: oai:DiVA.org:umu-20816DiVA: diva2:209624