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A predictive metabolomics evaluation of nutrition-modulated metabolic stress responses in human blood serum during the early recovery phase of strenuous physical exercise
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
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2009 (Engelska)Ingår i: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 8, nr 6, s. 2966-77Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

We have investigated whether postexercise ingestion of carbohydrates in combination with proteins generates a different systemic metabolic response, as compared to the sole ingestion of carbohydrate or water, in the early recovery phase following exercise. In addition, metabolic patterns related to fitness level were studied together with individual responses to nutritional modulation. Twenty-four male subjects were exposed to 90 min of ergometer-cycling. Each participant was subject to four identical test-sessions, including ingestion of one of four beverages (water, low-carbohydrate beverage, high-carbohydrate beverage, and low-carbohydrate−protein beverage (LCHO-P)) immediately after cycling. Blood was collected at six time points, one pre- and five postexercise. Extracted blood serum was subject to metabolomic characterization by gas chromatography/time-of-flight mass spectrometry (GC−TOF MS). Data was processed using hierarchical multivariate curve resolution (HMCR), and multivariate statistical analysis was carried out using orthogonal partial least-squares (OPLS). Predictive metabolomics, including predictive HMCR and OPLS classification, was applied to ensure efficient sample processing and validation of detected metabolic patterns. Separation of subjects in relation to ingested beverage was detected and interpreted. Pseudouridine was suggested as a novel marker for pro-anabolic effect following LCHO-P ingestion, which was supported by the detected decrease of the catabolic marker 3-methylhistidine. Separation of subjects according to fitness level was achieved, and nutritional modulation by LCHO-P was shown to improve the metabolic status of less fit subjects in the recovery phase. In addition, the potential of the methodology for detection of early signs of insulin resistance was also demonstrated.

Ort, förlag, år, upplaga, sidor
Washington: American Chemical Society , 2009. Vol. 8, nr 6, s. 2966-77
Nyckelord [en]
GC-MS, metabolomics, predictive metabolomics, chemometrics, human, exercise, serum, recovery, nutrition, pseudouridine
Nationell ämneskategori
Idrottsvetenskap
Identifikatorer
URN: urn:nbn:se:umu:diva-21143DOI: 10.1021/pr900081qPubMedID: 19317510OAI: oai:DiVA.org:umu-21143DiVA, id: diva2:210660
Tillgänglig från: 2009-04-03 Skapad: 2009-04-03 Senast uppdaterad: 2018-06-09Bibliografiskt granskad
Ingår i avhandling
1. Mapping the consequenses of physical exercise and nutrition on human health: A predictive metabolomics approach
Öppna denna publikation i ny flik eller fönster >>Mapping the consequenses of physical exercise and nutrition on human health: A predictive metabolomics approach
2011 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Human health is a complex and wide-ranging subject far beyond nutrition and physical exercise. Still, these factors have a huge impact on global health by their ability to prevent diseases and thus promote health. Thus, to identify health risks and benefits, it is necessary to reveal the underlying mechanisms of nutrition and exercise, which in many cases follows a complex chain of events. As a consequence, current health research is generating massive amounts of data from anthropometric parameters, genes, proteins, small molecules (metabolites) et cetera, with the intent to understand these mechanisms. For the study of health responses, especially related to physical exercise and nutrition, alterations in small molecules (metabolites) are in most cases immediate and located close to the phenotypic level and could therefore provide early signs of metabolic imbalances. Since there are roughly as many different responses to exercise and nutrients as there are humans, this quest is highly multifaceted and will benefit from an interpretation of treatment effects on a general as well as on an individual level. This thesis involves the application of chemometric methods to the study of global metabolic reactions, i.e. metabolomics, in a strategy coined predictive metabolomics. Via the application of predictive metabolomics an extensive hypothesis-free biological interpretation has been carried out of metabolite patterns in blood, acquired using gas chromatography-mass spectrometry (GC-MS), related to physical exercise, nutrition and diet, all in the context of human health. In addition, the chemometrics methodology have computational benefits concerning the extraction of relevant information from information-rich data as well as for interpreting general treatment effects and individual responses, as exemplified throughout this work. Health concerns all lifestages, thus this thesis presents a strategic framework in combination with comprehensive interpretations of metabolite patterns throughout life. This includes a broad range of human studies revealing metabolic patterns related to the impact of physical exercise, macronutrient modulation and different fitness status in young healthy males, short and long term dietary treatments in overweight post menopausal women as well as metabolic responses related to probiotics treatment and early development in infants. As a result, the studies included in the thesis have revealed metabolic patterns potentially indicative of an anti-catabolic response to macronutrients in the early recovery phase following exercise. Moreover, moderate differences in the metabolome associated with cardiorespiratory fitness level were detected, which could be linked to variation in the inflammatory and antioxidaive defense system. This work also highlighted mechanistic information that could be connected to dietary related weight loss in overweight and obese postmenopausal women in relation to short as well as long term dietary effects based on different macronutrient compositions. Finally, alterations were observed in metabolic profiles in relation to probiotics treatment in the second half of infancy, suggesting possible health benefits of probiotics supplementation at an early age.

 

Ort, förlag, år, upplaga, sidor
Umeå: Print och Media, 2011. s. 60
Nyckelord
Metabolomics, physical exercise, cardiorespiratory fitness nutrition, high protein and fat diet, Lactobacillus F19, probiotics, GC-MS, plasma, chemometrics, multivariate analysis statistical experimental design, design of experiments
Nationell ämneskategori
Annan medicinsk grundvetenskap
Forskningsämne
systemanalys; näringslära; molekylär medicin (medicinska vetenskaper)
Identifikatorer
urn:nbn:se:umu:diva-43844 (URN)978-91-7459-128-6 (ISBN)
Disputation
2011-06-03, KB3B1, KBC-huset, Umeå universitet, Umeå, 10:00 (Engelska)
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Anmärkning
Embargo until 2012-06-01Tillgänglig från: 2011-05-12 Skapad: 2011-05-12 Senast uppdaterad: 2018-06-08Bibliografiskt granskad

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Chorell, ElinAntti, HenrikSvensson, Michael

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