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Detection of occult tumour cells in lymph nodes of colorectal cancer patients using real-time quantitative RT-PCR for CEA and CK20 mRNAS
Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Surgery.
Department of Surgery, Helsingborgs Lasarett, Lund University, Helsingborg, Sweden.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Immunology/Immunchemistry.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Immunology/Immunchemistry.
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2004 (English)In: International Journal of Cancer, ISSN 0020-7136, E-ISSN 1097-0215, Vol. 111, no 1, 101-110 p.Article in journal (Refereed) Published
Abstract [en]

The purpose of our study was to develop specific, sensitive, objective assays for early detection of disseminated tumour cells in patients with colorectal cancer (CRC). Carcinoembryonic antigen (CEA) and cytokeratin 20 (CK20) were chosen as markers because they are selectively expressed in epithelial cells with maintained expression in CRC. Real-time quantitative RT-PCR assays with RNA copy standards were constructed. Regional lymph nodes were collected from patients with CRC (n = 51) and benign intestinal disease (n = 10). Results were compared to routine histopathology and anti-CEA immunohistochemistry. Lymph node levels of CEA and CK20 mRNA correlated strongly (p < 0.0001, r = 0.8). Lymph nodes from non-CRC patients had <0.01 CEA and <0.001 CK20 mRNA copies/18S rRNA unit. Lymph nodes from 3/6 Dukes' A, 17/26 Dukes' B, 10/10 Dukes' C and 7/9 Dukes' D patients had CEA mRNA levels above cut-off. Corresponding figures for CK20 mRNA were 3/6, 10/26, 9/10 and 5/9, respectively. CEA mRNA levels varied from 0.001 to 100 copies/18S rRNA unit in Dukes' A and B, and 50% of the Dukes' B patients had CEA mRNA levels within the range of Dukes' C patients. Three Dukes' B patients have died from CRC or developed distant metastases. All 3 had high CEA and CK20 mRNA levels. Determination of mRNA was superior to immunohistochemistry in showing CEA expression in lymph nodes. The present qRT-PCR assay for CEA mRNA seems to be a superior tool to identify individuals with disseminated tumour cells. Future extended studies will establish the clinically most relevant cut-off level.

Place, publisher, year, edition, pages
Geneve: International union against cancer , 2004. Vol. 111, no 1, 101-110 p.
Keyword [en]
colorectal neoplasm, carcinoembryonic antigen, cytokeratin 20, real-time quantitative RT-PCR, lymph node metastases, minimal residual cancer
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:umu:diva-21218DOI: 10.1002/ijc.20231PubMedID: 15185350OAI: oai:DiVA.org:umu-21218DiVA: diva2:211044
Available from: 2009-04-07 Created: 2009-04-07 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Prognostic factors in colorectal cancer: aspects of tumour dissemination
Open this publication in new window or tab >>Prognostic factors in colorectal cancer: aspects of tumour dissemination
2002 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Umeå: Umeå Universitet, 2002. 61 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 798
Keyword
Colorectal neoplasms, prognosis, lymph node metastases, disseminated tumour cells, cytokeratins, micrometastases, metalloproteinases, immune cells, real-time quantitative RT-PCR, carsinoembryonic antigen
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:umu:diva-94103 (URN)91-7305-264-7 (ISBN)
Public defence
2002-09-20, Tandläkarhögskolan, Sal B, 9 tr., Umeå universitet, Umeå, 09:00
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digitalisering@umu
Note

Diss. (sammanfattning) Umeå : Umeå universitet, 2002

Available from: 2014-11-19 Created: 2014-10-03 Last updated: 2015-04-10Bibliographically approved

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