Dominant IL-10 and TGF-beta mRNA expression in gammadeltaT cells of human early pregnancy decidua suggests immunoregulatory potential.
2002 (English)In: American journal of reproductive immunology (New York, N.Y. : 1989), ISSN 1046-7408, Vol. 48, no 1, 9-17 p.Article in journal (Refereed) Published
PROBLEM: To examine the cytokine gene expression in gammadeltaT-cells from human early pregnancy decidua. METHOD OF STUDY: The cytokine messenger RNA (mRNA) expression in isolated decidual T-cell receptor (TCR)gammadelta+/CD56+ and TCRgammadelta single positive cells was investigated with a panel of cytokine primers and probes selected to distinguish between T helper (Th)1, Th2, Th3 and regulatory T-cells (Tr1) type of immune response using real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: TCRgammadelta+/CD56+ cells express almost exclusively the immunosuppressive cytokines interleukin-10 (IL-10) and transforming growth factor (TGF)-beta. The TCRgammadelta single positive cells enhance their transcription of IL-10 and TGF-beta, compared with the TCRgammadelta+ CD56+ cells and additionally express mRNA for IL-1beta and IL-6. CONCLUSIONS: The present findings suggest that gammadeltaT cells in normal pregnancy create a cytokine milieu promoting immunotolerance to the fetus. We hypothesize that through the production of the immunosuppressive cytokines IL-10 and/or TGF-beta the gammadeltaT cells could function directly as regulatory T cells or induce the differentiation of Th0 TCRalphabeta+ cells into regulatory/suppresser cells.
Place, publisher, year, edition, pages
2002. Vol. 48, no 1, 9-17 p.
IdentifiersURN: urn:nbn:se:umu:diva-21510PubMedID: 12322898OAI: oai:DiVA.org:umu-21510DiVA: diva2:211356