Characterization of anandamide retention in synthetic liposomes
(English)Manuscript (Other academic)
Anandamide (AEA) is an endogenous ligand for cannabinoid receptors. Prior to termination of signalling activity AEA has to move across the plasma membrane to the reach its intracellular hydrolytic enzyme. The mechanism underlying this transport is under considerable debate. In the present study, we have examined the properties of AEA retention using synthetic large unilamellar liposomes (LUVs). Retention of AEA in LUVs was saturable, time- and temperature-dependent. Preincubation of LUVs with inhibitors of AEA uptake and metabolism did not decrease the tritium retention. These results mimic the “initial” cellular uptake of AEA and indeed argue against the need of a specific membrane transporter protein.
Liposome, endocannabinoid, anandamide, membrane retention
Pharmacology and Toxicology
Research subject biokemisk farmakologi
IdentifiersURN: urn:nbn:se:umu:diva-22170OAI: oai:DiVA.org:umu-22170DiVA: diva2:213028