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Magic-angle phosphorus NMR of functional mitochondria: in situ monitoring of lipid response under apoptotic-like stress
Umeå University, Faculty of Science and Technology, Department of Chemistry. (UMR 5248 Chimie et Biologie des Membranes et des Nanoobjets, Centre National de la Recherche Scientifique, Université Bordeaux 1, Pessac, France)
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0002-0546-7721
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0001-5900-7395
UMR 5248 Chimie et Biologie des Membranes et des Nanoobjets, Centre National de la Recherche Scientifique, Université Bordeaux 1, Pessac, France.
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2009 (English)In: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 23, no 9, 2872-2878 p.Article in journal (Refereed) Published
Abstract [en]

Using a noninvasive, solid-state magic-angle spinning nuclearmagnetic resonance (MAS NMR) approach, we track ex vivo thebehavior of individual membrane components in isolated, activemitochondria (model system: potato tubers) during physiologicalprocesses. The individual phosphatidylcholine (PC), phosphatidylethanolamine(PE), and cardiolipin (CL) membrane constituents were identifiedas distinct lines by applying MAS 31P NMR on extracted lipidmembranes. However, the CL NMR signal appeared to be very broadin functional mitochondria, indicating a tight complex formationwith membrane protein. Calcium stress induced severe membranedegradation without recovery of a single CL NMR resonance. Thissuggests that calcium overload destroys the outer mitochondrialmembrane and does not modify strongly the CL protein complexesin the inner membrane; a conclusion confirmed by respiratorycontrols. Conversely, mitochondrial membrane disruption on timedegradation or mechanical stress generates clearly visible identicalCL NMR signals, similar to those observed in rehydrated lipidextracts. Similarly, noninvasive based NMR tracking of lipidsin response to diverse physiological stimuli can easily be usedfor other organelles and whole living cells. Sani, M.-A., Keech,O., Gardeström, P., Dufourc, E. J., Gröbner, G. Magic-anglephosphorus NMR of functional mitochondria: in situ monitoringof lipid response under apoptotic-like stress.

Place, publisher, year, edition, pages
The Federation of American Societies for Experimental Biology , 2009. Vol. 23, no 9, 2872-2878 p.
Keyword [en]
respiration, oxidative stress, membrane integrity, cardiolipin, magnetic resonance, programmed cell death
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-22243DOI: 10.1096/fj.09-134114OAI: oai:DiVA.org:umu-22243DiVA: diva2:214130
Available from: 2009-05-04 Created: 2009-05-04 Last updated: 2017-12-13Bibliographically approved

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Sani, Marc-AntoineKeech, OlivierGardeström, PerGröbner, Gerhard
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Department of ChemistryDepartment of Plant PhysiologyUmeå Plant Science Centre (UPSC)
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